Wingren U, Enerbäck L
Histochem J. 1983 Jun;15(6):571-82. doi: 10.1007/BF01954148.
Mucosal mast cells of the gastrointestinal tract constitute a separate cell line within the mast cell system of the rat, differing in several respects from the classical connective tissue mast cells and, unlike the latter, requiring special fixation techniques for their demonstration. We have examined some histochemical properties of mucosal mast cells of the duodenum and compared them with connective tissue mast cells of the tongue or skin. The results indicate that the structural integrity of the granules of both types of mast cell is partly dependent on ionic linkages between glycosaminoglycan and protein. The so far unidentified glycosaminoglycan of mucosal mast cells appears to be more soluble than the heparin of connective tissue mast cells. The strongly fluorescent binding of Berberine to the granules of connective tissue mast cells and, depending on their content, of heparin is absent from mucosal mast cells, confirming previous findings which suggested that they contain a glycosaminoglycan with a lower degree of sulphation. Aldehyde fixation by routine procedures reversibly blocks the cationic dye binding of mucosal mast cell granules. The dye binding groups may be unmasked by trypsination or by long staining times of the order of several days. The results suggest that the blocking of staining by aldehydes is caused by a diffusion barrier of a protein nature. Mucosal and connective tissue mast cells thus differ with respect to the spatial arrangement of glycosaminoglycan and protein in their granules. As a result of the study a modified method for the demonstration of mucosal mast cells in tissue sections is described, based on normal formaldehyde fixation and staining in Toluidine Blue for a long time. It has some advantages over previous methods and preserves the structure of mucosal and connective tissue mast cells equally well.
胃肠道的黏膜肥大细胞在大鼠肥大细胞系统中构成一个独立的细胞系，在几个方面与经典的结缔组织肥大细胞不同，并且与后者不同的是，需要特殊的固定技术来显示它们。我们研究了十二指肠黏膜肥大细胞的一些组织化学特性，并将它们与舌或皮肤的结缔组织肥大细胞进行了比较。结果表明，两种类型肥大细胞颗粒的结构完整性部分取决于糖胺聚糖和蛋白质之间的离子键。黏膜肥大细胞中迄今未鉴定的糖胺聚糖似乎比结缔组织肥大细胞的肝素更易溶解。黄连素与结缔组织肥大细胞颗粒以及视肝素含量而定与肝素的强烈荧光结合在黏膜肥大细胞中不存在，这证实了先前的研究结果，即它们含有硫酸化程度较低的糖胺聚糖。常规程序的醛固定可逆地阻断黏膜肥大细胞颗粒的阳离子染料结合。染料结合基团可通过胰蛋白酶消化或几天的长时间染色来暴露。结果表明，醛对染色的阻断是由蛋白质性质的扩散屏障引起的。因此，黏膜肥大细胞和结缔组织肥大细胞在其颗粒中糖胺聚糖和蛋白质的空间排列方面存在差异。作为研究的结果，描述了一种在组织切片中显示黏膜肥大细胞的改良方法，该方法基于正常甲醛固定和长时间甲苯胺蓝染色。它比以前的方法有一些优点，并且能同样好地保存黏膜和结缔组织肥大细胞的结构。
