Morwinski R, Karsten U, Wallukat G
Max Delbrück Centre for Molecular Medicine, Berlin, Germany.
Agents Actions. 1994 Jun;41 Spec No:C39-40. doi: 10.1007/BF02007757.
Although the presence of mast cells in heart muscle is well documented, their possible presence in heart cell cultures has not yet been considered. In this paper we show for the first time that cultures from neonatal rat hearts contain mast cells in proportions which may exert physiological influences on heart muscle cells. Evidence is based on cytochemistry, immunocytochemistry with a monoclonal antibody (F2) specific for connective tissue mast cells of the rat, and direct estimations of both cellular histamine and histamine released into the culture medium. Since the number of nonmuscle cells immunoreactive with antibody F2 exceeded the number of cells reacting with conventional cytochemical stains for mast cells, a substantial proportion of the former may represent less differentiated (precursor) cells.
尽管心肌中肥大细胞的存在已有充分记录，但它们在心脏细胞培养物中可能存在的情况尚未得到考虑。在本文中，我们首次表明新生大鼠心脏的培养物中含有肥大细胞，其比例可能对心肌细胞产生生理影响。证据基于细胞化学、使用针对大鼠结缔组织肥大细胞的单克隆抗体（F2）进行的免疫细胞化学，以及对细胞组胺和释放到培养基中的组胺的直接测定。由于与抗体F2发生免疫反应的非肌肉细胞数量超过了与传统肥大细胞细胞化学染色反应的细胞数量，前者中的很大一部分可能代表分化程度较低的（前体）细胞。
