Zorić Lepsa, Aleksić Petar, Koraćević Darinka, Trajković Goran
Medicinski fakultet, Klinika za ocne bolesti, Kosovska Mitrovica.
Vojnosanit Pregl. 2005 Dec;62(12):909-13. doi: 10.2298/vsp0512909z.
BACKGROUND/AIM: Oxidative stress results from increased oxidative processes, decreased antioxidative protection, or both processes simultaneously. Photooxidative stress, as a form of oxidative stress, induced by the energy of solar radiation, today is considered as crucial in the age-related cataractogenesis. Other known and unknown, endogenous and egsogenous factors that contribute to the oxidative stress intensity, can influnce the cataract type and brunescence. Thus the oxidative stress intensity and its form might determine the cataract type and brunescence, and also make the efforts in cataract prevention more complex. Hence, the objective of the present paper was to investigate the current amount of antioxidative capacity in aqueous humour during the cataract genesis of different types and pigmentation of cataract.
Transversal review of 80 samples of humour aqueous obtained during extracapsular cataract extraction. Aqueouses were analyzed by tiobarbituric acid (TBA) method for the total antioxidant activity estimation, expressed as %iMDA, and by using 0.1 ml of aqueous.
The mixed type of cataract showed the statistically significantly lower values of the intensities of antioxidative protection in aqueous humour compared to cortical and nuclear cataracts (p < 0.001, respectively). Between pure nuclear and cortical cataracts we found the small differences of the investigated parameter, but they pointed to the decreased level of antioxidative protection, i.e. the increased intensity of the aqueous humour oxidative stress in the cortical cataract type. A significant correlation betweer the cortical cataract maturation and the %iMDA (p < 0.05) was found.
The role of the oxidative stress, here expressed as the antioxidative capacity of aqueous humour, could not be the same for all the cataract types. The lower level of antioxidative protection of aqueous in brunescent and mixed cataracts may point to the higher intensity of oxidative stress in those cataract types. The correlation betweer the cortical cataract maturity and %iMDA points to the significant decrease of the aqueous antioxidative protection in the cataract progression.
背景/目的：氧化应激源于氧化过程增加、抗氧化保护作用降低或这两个过程同时发生。光氧化应激作为氧化应激的一种形式，由太阳辐射能量诱导产生，如今被认为在年龄相关性白内障的发生过程中起着关键作用。其他已知和未知的、内源性和外源性因素会影响氧化应激强度，进而可能影响白内障的类型和棕色化程度。因此，氧化应激强度及其形式可能决定白内障的类型和棕色化程度，也使得白内障预防工作变得更加复杂。因此，本文的目的是研究不同类型白内障发生及不同色素沉着的白内障患者房水中当前的抗氧化能力。
对80例白内障囊外摘除术中获取的房水样本进行横向回顾性研究。采用硫代巴比妥酸（TBA）法分析房水，以评估总抗氧化活性，用%iMDA表示，每份房水样本用量为0.1 ml。
与皮质性和核性白内障相比，混合型白内障房水中抗氧化保护强度在统计学上显著较低（分别为p < 0.001）。在纯核性和皮质性白内障之间，我们发现所研究参数存在细微差异，但这些差异表明皮质性白内障类型中房水抗氧化保护水平降低，即房水氧化应激强度增加。发现皮质性白内障成熟度与%iMDA之间存在显著相关性（p < 0.05）。
氧化应激（此处以房水的抗氧化能力表示）对所有类型的白内障作用可能并不相同。棕色化和混合型白内障房水抗氧化保护水平较低，可能表明这些类型白内障的氧化应激强度较高。皮质性白内障成熟度与%iMDA之间的相关性表明，在白内障进展过程中房水抗氧化保护显著降低。
