Ram Prassad V V S, Nirmala N Rao, Kotian M S
Department of Oral Pathology and Microbiology, Manipal College of Dental Sciences, Affiliated to Manipal Academy of Higher Education, India.
Indian J Dent Res. 2005 Jan-Mar;16(1):6-11.
Recent progress in understanding the biology of keratins together with the development of monoclonal antibodies to individual keratin proteins provide the foundation for studying the keratin expression in normal and pathological oral epithelia. Cytokeratin (CK) alterations have been reported in carcinomas and these have been associated with specific aspects of tumour behaviour. Immunohisto-chemistry with monospecific CK19 antibody was used to study the expression pattern in normal mucosa, dysplasias, and oral squamous cell carcinomas (OSCC). In non-keratinzed normal mucosa, CK19 was detected in the basal cell layer, while in dysplasias (diagnosed in H and E stained sections, mild-severe) stained strongly for CK 19 in the basal and supra basal cell layers indicating layer specificity for CK 19 expression. In OSCC, in the number of CK19 labelled cells increased from well to poorly differentiated tumour. Thus the results of the present study indicate an alteration in synthesis of keratin proteins when exposed to carcinogens.
近年来，在角蛋白生物学研究方面取得的进展以及针对单个角蛋白蛋白的单克隆抗体的开发，为研究正常和病理状态下口腔上皮中的角蛋白表达奠定了基础。细胞角蛋白（CK）改变已在癌症中被报道，并且这些改变与肿瘤行为的特定方面相关。使用单特异性CK19抗体进行免疫组织化学研究，以探讨其在正常黏膜、发育异常组织和口腔鳞状细胞癌（OSCC）中的表达模式。在非角化正常黏膜中，CK19在基底细胞层被检测到，而在发育异常组织（在苏木精和伊红染色切片中诊断为轻度至重度）中，基底和基底上层细胞层中CK19染色强烈，表明CK19表达具有层特异性。在OSCC中，CK19标记细胞的数量从高分化肿瘤到低分化肿瘤逐渐增加。因此，本研究结果表明，暴露于致癌物时角蛋白蛋白的合成会发生改变。
