Lee Jeeyoung, Kim Heechul, Ahn Meejung, Min Do Sik, Shin Taekyun
Department of Veterinary Medicine, Cheju National University, Aradong 1, Jeju 690-756, South Korea.
Neurosci Lett. 2006;397(1-2):44-7. doi: 10.1016/j.neulet.2005.11.051. Epub 2005 Dec 22.
The expression of phospholipase D1 (PLD1) was examined in the retinas of pigs. Western blot analysis detected the expression of PLD1 in the retinas of 1-day-old piglets and showed that it was enhanced in the retinas of 2 years old adult pigs. Immunohistochemically, PLD1 was mainly immunostained in ganglion cell bodies in the ganglion cell layer, in some radial processes of Muller cells in the retinal layer and in the inner and outer segments of the rod and cone layer in newborn and adult pigs, but not in astrocytic bundles in nerve fiber layers. The immunoreactivity of PLD1 in the radial processes of Muller cells across the retinal layers was enhanced in adult pig retinas compared to those of newborn piglets. This was the first demonstration to show that PLD1 is constitutively expressed in the retina of pigs, implying that retinal PLD1 expression is enhanced in radial fibers of Muller cells with age. This finding suggests that PLD1 plays an important role in signal transduction of glial cells and neuronal cells in the retina.
研究了磷脂酶D1（PLD1）在猪视网膜中的表达情况。蛋白质免疫印迹分析检测到1日龄仔猪视网膜中有PLD1表达，且显示其在2岁成年猪视网膜中表达增强。免疫组织化学结果表明，在新生猪和成年猪中，PLD1主要在神经节细胞层的神经节细胞胞体、视网膜层中Muller细胞的一些放射状突起以及视杆和视锥层的内节和外节中免疫染色，但在神经纤维层的星形细胞束中未检测到。与新生仔猪相比，成年猪视网膜中跨视网膜层的Muller细胞放射状突起中PLD1的免疫反应性增强。这是首次证明PLD1在猪视网膜中组成性表达，这意味着随着年龄增长，视网膜中PLD1在Muller细胞的放射状纤维中的表达增强。这一发现表明PLD1在视网膜中神经胶质细胞和神经元细胞的信号转导中起重要作用。
