Kim Jihoon, Moon Changjong, Ahn Meejung, Joo Hong-Gu, Jin Jae-Kwang, Shin Taekyun
Department of Veterinary Anatomy, College of Veterinary Medicine, Jeju National University, Jeju, South Korea.
Mol Vis. 2009 Sep 26;15:1971-6.
The differential level and localization of galectin-3 protein were examined in the retinas of two-day-old pigs and six-month-old pigs.
The retinas sampled from two-day-old and six-month-old pigs were analyzed by western blot and immunohistochemistry.
western blot analysis detected galectin-3 in both age groups, although the levels were significantly higher in six-month-old pigs. Immunohistochemical staining showed that galectin-3 was localized in the retinas of both two-day-old pigs and six-month-old pigs; the galectin-3 immunostaining was more intense in the six-month-old pig retina, as shown in the western blot analysis. Galectin-3 was expressed in glial cells, particularly in glutamine synthetase-positive Müller cells and their processes, across all retina layers in both age groups; however, it was not found in ganglion cells of the ganglion cell layer or neuronal cells of the inner and outer nuclear cell layers in either age group.
This is the first demonstration that galectin-3 is detected in the retinas of two-day-old pigs and that the expression in Müller cells increases with postnatal development.
检测2日龄猪和6月龄猪视网膜中半乳糖凝集素-3蛋白的差异水平及定位。
采用蛋白质免疫印迹法和免疫组织化学法对2日龄和6月龄猪的视网膜样本进行分析。
蛋白质免疫印迹分析在两个年龄组中均检测到半乳糖凝集素-3，尽管6月龄猪中的水平显著更高。免疫组织化学染色显示，半乳糖凝集素-3定位于2日龄猪和6月龄猪的视网膜中；如蛋白质免疫印迹分析所示，半乳糖凝集素-3免疫染色在6月龄猪视网膜中更强。在两个年龄组的所有视网膜层中，半乳糖凝集素-3均在神经胶质细胞中表达，尤其是在谷氨酰胺合成酶阳性的米勒细胞及其突起中；然而，在两个年龄组的神经节细胞层的神经节细胞或内核层和外核层的神经元细胞中均未发现。
这是首次证明在2日龄猪的视网膜中检测到半乳糖凝集素-3，且其在米勒细胞中的表达随出生后发育而增加。
