Tendler Yevgeny, Panshin Alex, Weisinger Gary, Zinder Oren
Department of Clinical Biochemistry, Rambam Medical Center, Haifa, Israel.
Exp Eye Res. 2006 Apr;82(4):674-81. doi: 10.1016/j.exer.2005.09.005. Epub 2005 Dec 22.
The tumour suppressor gene p53 encodes a phosphoprotein involved in the control of cell growth. It's expression and function have been documented in malignancy, apoptosis and the aging processes. Recently, p53 expression has been demonstrated in normal murine tissues, including whole eye. Currently, we intend to map and to characterize p53 expression in the normal cornea across different species. To do this, eyes of animals were enucleated after sacrifice by CO(2) narcosis and then p53 expression in whole eyes (cornea) was mapped by indirect immunohistochemical staining techniques using the anti-p53 monoclonal antibodies PAb 248, PAb 421 and PAb 240 (alternatively called mAb 248, mAb 421 and mAb 240, respectively). Additionally, eyes were freshly dissected to separate the corneas, for quantitating p53 expression, using Western blot analysis. We found strong cytoplasmic p53 expression in the corneal epithelium of various vertebrate species by immunohistochemistry and by Western analysis. High levels of cytoplasmic p53 protein were normally found in normal corneal epithelium of various vertebrate species. Hence, these data may indicate that p53 may have a new evolutionary significant function in the eye.
肿瘤抑制基因p53编码一种参与细胞生长控制的磷蛋白。其表达和功能已在恶性肿瘤、细胞凋亡及衰老过程中得到记载。最近，已证实在包括全眼在内的正常鼠组织中有p53表达。目前，我们打算绘制并描述不同物种正常角膜中p53的表达情况。为此，通过二氧化碳麻醉处死动物后摘除眼球，然后使用抗p53单克隆抗体PAb 248、PAb 421和PAb 240（也分别称为mAb 248、mAb 421和mAb 240），通过间接免疫组织化学染色技术绘制全眼（角膜）中p53的表达情况。此外，将眼球新鲜解剖分离出角膜，用蛋白质印迹分析法定量p53表达。通过免疫组织化学和蛋白质印迹分析，我们在各种脊椎动物物种的角膜上皮中发现了强烈的细胞质p53表达。在各种脊椎动物物种的正常角膜上皮中通常可发现高水平的细胞质p53蛋白。因此，这些数据可能表明p53在眼中可能具有新的具有进化意义的功能。
