Queiroz Conceição, Silva Tânia Correia, Alves Venâncio A F, Villa Luisa L, Costa Maria Cecília, Travassos Ana Gabriela, Filho José Bouzas Araújo, Studart Eduardo, Cheto Tatiana, de Freitas Luiz Antonio R
Department of Gynecology and Obstetrics, Medical School, and Mathematics and Statistic School, Federal University of Bahia, Salvador, Bahia, Brazil.
Pathol Res Pract. 2006;202(2):77-83. doi: 10.1016/j.prp.2005.08.012. Epub 2005 Dec 22.
An immunohistochemical analysis with monoclonal antibody p16(INK4a) was performed in formalin-fixed, paraffin-embedded samples of 60 cases. The aim was to investigate in biopsies the expression of p16(INK4a) of normal uterine cervical tissue, pre-cancerous and cancerous lesions, and their relation with human papilloma virus (HPV) and HIV status. Three parameters were evaluated: percentage of p16(INK4a) positive cells, reaction intensity, and cell staining pattern. All of these parameters were statistically different when compared among different histological groups. However, logistic regression model showed that the reaction intensity was the best indicator of the expression of p16(INK4a). This expression increases from normal to invasive squamous carcinoma. Sixty-six percent of the patients with CIN grade 1 (CIN1) expressed p16(INK4a) (all these cases were infected with high risk HPV). Our study supports the hypothesis that p16(INK4a) expression in pre-cancerous lesions and cancers can be used to identify HPV-transformed cells. Of great interest for routine diagnostic use is the fact that immunohistochemical testing for p16(INK4a) seems to be capable of identifying HPV-positive cells and potentially recognizing those lesions with an increased risk of progression to high-grade lesions.
对60例福尔马林固定、石蜡包埋样本进行了单克隆抗体p16（INK4a）免疫组化分析。目的是研究活检中正常子宫颈组织、癌前病变和癌性病变中p16（INK4a）的表达情况，及其与人乳头瘤病毒（HPV）和HIV状态的关系。评估了三个参数：p16（INK4a）阳性细胞百分比、反应强度和细胞染色模式。在不同组织学组之间比较时，所有这些参数均有统计学差异。然而，逻辑回归模型显示反应强度是p16（INK4a）表达的最佳指标。这种表达从正常组织到浸润性鳞状细胞癌逐渐增加。66%的1级子宫颈上皮内瘤变（CIN1）患者表达p16（INK4a）（所有这些病例均感染高危HPV）。我们的研究支持以下假设，即癌前病变和癌症中的p16（INK4a）表达可用于识别HPV转化细胞。对于常规诊断用途而言，一个非常有趣的事实是，p16（INK4a）免疫组化检测似乎能够识别HPV阳性细胞，并有可能识别那些进展为高级别病变风险增加的病变。
