Zhang Caining, Zhang Xiaozhe, Zhang Yan, Xu Qing, Xiao Hongbin, Liang Xinmiao
Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, P.R. China.
J Ethnopharmacol. 2006 Apr 21;105(1-2):223-8. doi: 10.1016/j.jep.2005.10.029. Epub 2005 Dec 27.
The estrogenic activity of traditional Chinese herb-Polygonum cuspidatum Sieb. et Zucc. was investigated by a recombinant yeast screening (YES) assay. Anthraquinones are the main components in the plant, of which emodin is the most abundant one. The ethyl acetate fraction of the ethanol extract of Polygonum cuspidatum was separated on a silica gel TLC plate and seven sub-fractions were collected. The results of bioassay demonstrated that Hzs1 and Hzs6 showed higher estrogenic activities than that of others and the potency of these two compounds were approximately 10(-4) g/L and 10(-3) g/L, respectively. HPLC analysis was performed to determine the activities and the active components. Combining the results of HPLC analysis and estrogenic activity test by YES led to the conclusion that an unknown bioactive compound might exist in the extraction of Polygonum cuspidatum.
采用重组酵母筛选（YES）试验对传统中药虎杖的雌激素活性进行了研究。蒽醌类是该植物的主要成分，其中大黄素含量最为丰富。将虎杖乙醇提取物的乙酸乙酯部分在硅胶TLC板上进行分离，收集到7个亚组分。生物测定结果表明，Hzs1和Hzs6的雌激素活性高于其他组分，这两种化合物的效价分别约为10(-4) g/L和10(-3) g/L。进行HPLC分析以确定活性和活性成分。结合HPLC分析结果和YES雌激素活性测试得出结论，虎杖提取物中可能存在一种未知的生物活性化合物。
