VraSR双组分调节系统及其在金黄色葡萄球菌中细胞壁抗菌药物诱导pbp2和vraSR表达中的作用。
VraSR two-component regulatory system and its role in induction of pbp2 and vraSR expression by cell wall antimicrobials in Staphylococcus aureus.
作者信息
Yin Shaohui, Daum Robert S, Boyle-Vavra Susan
机构信息
Department of Pediatrics, The University of Chicago, 5841 S. Maryland Ave., MC 6054, Chicago, Illinois 60637, USA.
出版信息
Antimicrob Agents Chemother. 2006 Jan;50(1):336-43. doi: 10.1128/AAC.50.1.336-343.2006.
Abstract
The VraS/VraR two-component system (VraSR) regulates transcriptional induction of penicillin-binding protein 2 (encoded by pbp2) by vancomycin in Staphylococcus aureus. We have now defined the vraSR operon and determined that its induction by beta-lactams as well as by vancomycin is autoregulated. Induction of the pbp2 and vraSR operons by beta-lactams and related compounds within 1 hour after exposure to the antimicrobials was dependent on vraS. However, when a disk diffusion assay that can detect induction of genes over an extended time period was used, induction of the pbp2 operon was mediated by some beta-lactams, including oxacillin; this induction was independent of vraS.
摘要
VraS/VraR双组分系统（VraSR）可调节金黄色葡萄球菌中万古霉素对青霉素结合蛋白2（由pbp2编码）的转录诱导。我们现已确定了vraSR操纵子，并确定其受β-内酰胺类药物以及万古霉素的诱导是自动调节的。暴露于抗菌药物后1小时内，β-内酰胺类药物及相关化合物对pbp2和vraSR操纵子的诱导依赖于vraS。然而，当使用能够在较长时间段内检测基因诱导的纸片扩散试验时，pbp2操纵子的诱导是由一些β-内酰胺类药物介导的，包括苯唑西林；这种诱导不依赖于vraS。
相似文献
1
VraSR two-component regulatory system and its role in induction of pbp2 and vraSR expression by cell wall antimicrobials in Staphylococcus aureus.
Antimicrob Agents Chemother. 2006 Jan;50(1):336-43. doi: 10.1128/AAC.50.1.336-343.2006.
2
The VraS/VraR two-component regulatory system required for oxacillin resistance in community-acquired methicillin-resistant Staphylococcus aureus.
FEMS Microbiol Lett. 2006 Sep;262(2):163-71. doi: 10.1111/j.1574-6968.2006.00384.x.
3
Two-component system VraSR positively modulates the regulation of cell-wall biosynthesis pathway in Staphylococcus aureus.
Mol Microbiol. 2003 Aug;49(3):807-21. doi: 10.1046/j.1365-2958.2003.03599.x.
4
DNA-binding activity of the vancomycin resistance associated regulator protein VraR and the role of phosphorylation in transcriptional regulation of the vraSR operon.
Biochemistry. 2009 Jun 23;48(24):5592-601. doi: 10.1021/bi900478b.
5
Chromatin immunoprecipitation identifies genes under direct VraSR regulation in Staphylococcus aureus.
Can J Microbiol. 2012 Jun;58(6):703-8. doi: 10.1139/w2012-043. Epub 2012 May 9.
6
Role of VraSR in antibiotic resistance and antibiotic-induced stress response in Staphylococcus aureus.
Antimicrob Agents Chemother. 2006 Oct;50(10):3424-34. doi: 10.1128/AAC.00356-06.
7
A close-up view of the VraSR two-component system. A mediator of Staphylococcus aureus response to cell wall damage.
J Biol Chem. 2008 May 2;283(18):12354-64. doi: 10.1074/jbc.M710010200. Epub 2008 Mar 7.
8
Roles of DNA sequence and sigma A factor in transcription of the vraSR operon.
J Bacteriol. 2012 Jan;194(1):61-71. doi: 10.1128/JB.06143-11. Epub 2011 Oct 21.
9
Transcriptional profiling of the two-component regulatory system VraSR in Staphylococcus aureus with low-level vancomycin resistance.
Int J Antimicrob Agents. 2016 May;47(5):362-7. doi: 10.1016/j.ijantimicag.2016.02.003. Epub 2016 Mar 12.
10
Mutational analyses of open reading frames within the vraSR operon and their roles in the cell wall stress response of Staphylococcus aureus.
Antimicrob Agents Chemother. 2011 Apr;55(4):1391-402. doi: 10.1128/AAC.01213-10. Epub 2011 Jan 10.
引用本文的文献
1
Insights into Kinases of ESKAPE Pathogens for Therapeutic Interventions.
Cardiovasc Hematol Agents Med Chem. 2024;22(3):276-297. doi: 10.2174/0118715257267497231128093529.
2
The two-component regulatory systems GraRS and SrrAB mediate susceptibility to Pep5 produced by clinical isolate of .
Appl Environ Microbiol. 2024 Jul 24;90(7):e0030024. doi: 10.1128/aem.00300-24. Epub 2024 Jun 4.
3
Transcriptomic analysis of cell envelope inhibition by prodigiosin in methicillin-resistant .
Front Microbiol. 2024 Jan 22;15:1333526. doi: 10.3389/fmicb.2024.1333526. eCollection 2024.
4
NH125 Sensitizes Staphylococcus aureus to Cell Wall-Targeting Antibiotics through the Inhibition of the VraS Sensor Histidine Kinase.
Microbiol Spectr. 2023 Jun 15;11(3):e0486122. doi: 10.1128/spectrum.04861-22. Epub 2023 May 25.
5
Vancomycin Resistance in and .
Microorganisms. 2022 Dec 21;11(1):24. doi: 10.3390/microorganisms11010024.
6
Revisiting the Role of VraTSR in Response to Cell Wall-Targeting Antibiotics.
J Bacteriol. 2022 Aug 16;204(8):e0016222. doi: 10.1128/jb.00162-22. Epub 2022 Jul 13.
7
Antibiotic Exposure Leads to Reduced Phage Susceptibility in Vancomycin Intermediate Staphylococcus aureus (VISA).
Antimicrob Agents Chemother. 2022 Jul 19;66(7):e0224721. doi: 10.1128/aac.02247-21. Epub 2022 Jun 16.
8
Brominated Carbazole with Antibiotic Adjuvant Activity Displays Pleiotropic Effects in MRSA's Transcriptome.
ACS Chem Biol. 2022 May 20;17(5):1239-1248. doi: 10.1021/acschembio.2c00168. Epub 2022 Apr 25.
9
The physiology and genetics of bacterial responses to antibiotic combinations.
Nat Rev Microbiol. 2022 Aug;20(8):478-490. doi: 10.1038/s41579-022-00700-5. Epub 2022 Mar 3.
10
Two-Component Systems of : Signaling and Sensing Mechanisms.
Genes (Basel). 2021 Dec 23;13(1):34. doi: 10.3390/genes13010034.
本文引用的文献
1
Penicillin-binding protein 2 is essential for expression of high-level vancomycin resistance and cell wall synthesis in vancomycin-resistant Staphylococcus aureus carrying the enterococcal vanA gene complex.
Antimicrob Agents Chemother. 2004 Dec;48(12):4566-73. doi: 10.1128/AAC.48.12.4566-4573.2004.
2
Regulation of the expression of cell wall stress stimulon member gene msrA1 in methicillin-susceptible or -resistant Staphylococcus aureus.
Antimicrob Agents Chemother. 2004 Aug;48(8):3057-63. doi: 10.1128/AAC.48.8.3057-3063.2004.
3
Genome-wide transcriptional profiling of the response of Staphylococcus aureus to cell-wall-active antibiotics reveals a cell-wall-stress stimulon.
Microbiology (Reading). 2003 Oct;149(Pt 10):2719-2732. doi: 10.1099/mic.0.26426-0.
4
Two-component system VraSR positively modulates the regulation of cell-wall biosynthesis pathway in Staphylococcus aureus.
Mol Microbiol. 2003 Aug;49(3):807-21. doi: 10.1046/j.1365-2958.2003.03599.x.
5
Transcriptional induction of the penicillin-binding protein 2 gene in Staphylococcus aureus by cell wall-active antibiotics oxacillin and vancomycin.
Antimicrob Agents Chemother. 2003 Mar;47(3):1028-36. doi: 10.1128/AAC.47.3.1028-1036.2003.
6
Development of vancomycin and lysostaphin resistance in a methicillin-resistant Staphylococcus aureus isolate.
J Antimicrob Chemother. 2001 Nov;48(5):617-25. doi: 10.1093/jac/48.5.617.
7
An acquired and a native penicillin-binding protein cooperate in building the cell wall of drug-resistant staphylococci.
Proc Natl Acad Sci U S A. 2001 Sep 11;98(19):10886-91. doi: 10.1073/pnas.191260798. Epub 2001 Aug 21.
8
Whole genome sequencing of meticillin-resistant Staphylococcus aureus.
Lancet. 2001 Apr 21;357(9264):1225-40. doi: 10.1016/s0140-6736(00)04403-2.
9
Identification of the up- and down-regulated genes in vancomycin-resistant Staphylococcus aureus strains Mu3 and Mu50 by cDNA differential hybridization method.
Biochem Biophys Res Commun. 2000 Mar 16;269(2):485-90. doi: 10.1006/bbrc.2000.2277.
10
Cloning, characterization, and inactivation of the gene pbpC, encoding penicillin-binding protein 3 of Staphylococcus aureus.
J Bacteriol. 2000 Feb;182(4):1074-9. doi: 10.1128/JB.182.4.1074-1079.2000.
Zotero 插件