Sohn Ho-Yong, Kum Eun-Joo, Kwon Gi-Seok, Jin Ingnyol, Adams Claire A, Kuriyama Hiroshi
Department of Food and Nutrition, Andong National University, Korea.
Biosci Biotechnol Biochem. 2005 Dec;69(12):2450-4. doi: 10.1271/bbb.69.2450.
The role of glutathione (GSH) and its homeodynamics during respiratory oscillation of Saccharomyces cerevisiae were investigated. Pulse injection of thiol redox modifying agents, such as diethylmaleate, N-ethylmaleimide, DL-butione-[S,R]-sulfoxamine, or 5-nitro-2-furaldehyde into the culture perturbed oscillation, although the degree of perturbation varied. Analysis of the expression profiles of GSH1 and GLR1, the activities of glutathione reductase, oscillations in cysteine and GSH concentrations, and the chemostat culture of the GLR1 disruptant indicated that GLR1 plays an essential role in the homeodynamics of GSH and the regulation of H(2)S production.
研究了谷胱甘肽（GSH）及其内环境稳定动力学在酿酒酵母呼吸振荡过程中的作用。向培养物中脉冲注射硫醇氧化还原修饰剂，如马来酸二乙酯、N-乙基马来酰亚胺、DL-丁硫氨酸-[S,R]-亚砜胺或5-硝基-2-糠醛，虽干扰程度不同，但均会扰乱振荡。对GSH1和GLR1的表达谱、谷胱甘肽还原酶活性、半胱氨酸和GSH浓度的振荡以及GLR1破坏株的恒化器培养分析表明，GLR1在GSH的内环境稳定动力学和H₂S产生的调节中起重要作用。
