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Photochemical cross-linking of the anticodon loop of yeast tRNA(Phe) to 30S-subunit protein S7 at the ribosomal A and P sites.

作者信息

Sylvers L A, Kopylov A M, Wower J, Hixson S S, Zimmermann R A

机构信息

Program in Molecular and Cellular Biology, University of Massachusetts, Amherst 01003.

出版信息

Biochimie. 1992 Apr;74(4):381-9. doi: 10.1016/0300-9084(92)90116-v.

DOI:10.1016/0300-9084(92)90116-v
PMID:1637863
Abstract

Yeast tRNA(Phe), containing the photoreactive nucleoside 2-azidoadenosine at position 37 within the anticodon loop, has been cross-linked to the aminoacyl-tRNA (A) and peptidyl-tRNA (P) binding sites of the Escherichia coli ribosome. The 30S subunit was exclusively labeled in each case, and cross-linking occurred to both protein and 16S rRNA. Electrophoretic and immunological analyses demonstrated that S7 was the only 30S-subunit protein covalently attached to the tRNA. However, digestion of the A and P site-labeled S7 with trypsin revealed a unique pattern of cross-linked peptide(s) at each site. Thus, while the anticodon loop of tRNA is in close proximity to protein S7 at both the A and P sites, it neighbors a different portion of the protein molecule in each. The placement of the aminoacyl- and peptidyl-tRNA binding sites is discussed in relationship to recent models of the 30S ribosomal subunit.

摘要

相似文献

1
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2
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引用本文的文献

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A new technique for the characterization of long-range tertiary contacts in large RNA molecules: insertion of a photolabel at a selected position in 16S rRNA within the Escherichia coli ribosome.一种用于表征大型RNA分子中长程三级接触的新技术：在大肠杆菌核糖体的16S rRNA的选定位置插入光标记。
Nucleic Acids Res. 1997 Jun 15;25(12):2266-73. doi: 10.1093/nar/25.12.2266.
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Transfer RNA docking pair model in the ribosomal pre- and post-translocational states.
核糖体转位前后状态下的转运RNA对接配对模型。
Nucleic Acids Res. 1997 Mar 15;25(6):1254-64. doi: 10.1093/nar/25.6.1254.
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The ribosomal neighbourhood of the central fold of tRNA: cross-links from position 47 of tRNA located at the A, P or E site.转运RNA中心折叠区的核糖体邻域：来自位于A、P或E位点的转运RNA第47位的交联。
Nucleic Acids Res. 1995 Nov 25;23(22):4635-41. doi: 10.1093/nar/23.22.4635.
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Cloning and characterisation of the gene encoding the ribosomal protein S5 (also known as rp14, S2, YS8) of Saccharomyces cerevisiae.酿酒酵母核糖体蛋白S5（也称为rp14、S2、YS8）编码基因的克隆与鉴定
Nucleic Acids Res. 1995 Nov 25;23(22):4616-9. doi: 10.1093/nar/23.22.4616.
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