Hardesty B, Odom O W, Picking W
Department of Chemistry and Biochemistry, University of Texas, Austin 78712.
Biochimie. 1992 Apr;74(4):391-401. doi: 10.1016/0300-9084(92)90117-w.
Five different fluorescence phenomena are considered in relation to their use to study the structure and function of ribosomes. These are: quantum yield or emission intensity; emission wavelength maximum; fluorescence anisotropy; collisional quenching; and nonradiative energy transfer. Results from a number of studies in which these techniques were used are described and summarized in relation to the movement and conformation of tRNA, the nascent peptide, and mRNA in a ribosome during the reaction steps of peptide elongation.
本文考虑了五种不同的荧光现象，探讨了它们在研究核糖体结构和功能方面的应用。这五种现象分别是：量子产率或发射强度；最大发射波长；荧光各向异性；碰撞猝灭；以及非辐射能量转移。文中描述并总结了多项研究的结果，这些研究运用了上述技术，涉及肽链延伸反应步骤中核糖体内部tRNA、新生肽和mRNA的移动与构象。
