Ihalin Riikka, Karched Maribasappa, Eneslätt Kjell, Asikainen Sirkka
Division of Oral Microbiology, Umeå University, Umeå SE-90185, Sweden.
J Chromatogr B Analyt Technol Biomed Life Sci. 2006 Feb 2;831(1-2):116-25. doi: 10.1016/j.jchromb.2005.11.052. Epub 2005 Dec 27.
Peptidoglycan-associated lipoprotein (PAL) is a highly conserved structural outer membrane protein among Gram-negative bacteria. In some species, it is proinflammatory and released extracellularly. We purified a newly identified PAL (AaPAL) of a periodontal pathogen Actinobacillus actinomycetemcomitans by using AaPAL antipeptide antibodies coupled to immunoaffinity chromatography column. No protein impurities originating in A. actinomycetemcomitans were found in the final product. Sera from patients infected by A. actinomycetemcomitans recognized the purified AaPAL. The present purification method seems to be suitable for isolation of AaPAL and probably PALs of other bacterial species, and applicable in studies investigating proinflammatory mechanisms of A. actinomycetemcomitans.
肽聚糖相关脂蛋白（PAL）是革兰氏阴性菌中一种高度保守的结构外膜蛋白。在某些物种中，它具有促炎作用并释放到细胞外。我们通过使用与免疫亲和色谱柱偶联的AaPAL抗肽抗体，纯化了牙周病原体伴放线放线杆菌新鉴定的PAL（AaPAL）。最终产物中未发现源自伴放线放线杆菌的蛋白质杂质。伴放线放线杆菌感染患者的血清识别纯化的AaPAL。目前的纯化方法似乎适用于AaPAL以及其他细菌物种的PAL的分离，并可应用于研究伴放线放线杆菌的促炎机制。
