Paul-Satyaseela Maneesh, Karched Maribasappa, Bian Zhao, Ihalin Riikka, Borén Thomas, Arnqvist Anna, Chen Casey, Asikainen Sirkka
Oral Microbiology, Institute of Dentistry, Umeå University, SE-90187 Umeå, Sweden.
Microbiology and Tumor Biology Center, Karolinska Hospital, Stockholm, Sweden.
J Med Microbiol. 2006 Jul;55(Pt 7):931-942. doi: 10.1099/jmm.0.46470-0.
In a search for novel bioactive cell surface structures of periodontal pathogens, it was found that sera from two patients with Actinobacillus actinomycetemcomitans-associated infections reacted strongly at 17 kDa on immunoblots of A. actinomycetemcomitans outer-membrane protein (OMP) preparations. The 17 kDa antigen was also recognized by anti-CsgA (Escherichia coli curli major subunit) antibody. The 17 kDa A. actinomycetemcomitans protein was identified as peptidoglycan-associated lipoprotein (PAL; AaPAL) by two-dimensional immunoblotting and subsequent sequence analysis by mass spectrometry and bioinformatics tools. AaPAL was an OMP and a lipoprotein, and it had an OmpA-like domain. In a group of middle-aged subjects (n = 26), serum reactivity to AaPAL was associated with the presence of periodontitis but not with the oral detection of A. actinomycetemcomitans. Both human sera and rabbit antisera against three different types of antigens, the gel-purified AaPAL, A. actinomycetemcomitans whole-cell antigens, and CsgA, recognized putative PALs of oral haemophili in addition to AaPAL. The results demonstrated that the novel AaPAL is a conserved bacterial lipoprotein. It is expressed in vivo and is strongly immunoreactive. The antigenic cross-reactivity found between AaPAL and oral haemophili may enhance local and systemic immuno-inflammatory reactions in periodontitis.
在寻找牙周病原体新的生物活性细胞表面结构的过程中，发现两名伴放线放线杆菌相关感染患者的血清在伴放线放线杆菌外膜蛋白（OMP）制剂的免疫印迹上于17 kDa处有强烈反应。17 kDa抗原也被抗CsgA（大肠杆菌卷曲菌毛主要亚基）抗体识别。通过二维免疫印迹以及随后的质谱和生物信息学工具进行的序列分析，将伴放线放线杆菌的17 kDa蛋白鉴定为肽聚糖相关脂蛋白（PAL；AaPAL）。AaPAL是一种外膜蛋白和脂蛋白，并且具有类似OmpA的结构域。在一组中年受试者（n = 26）中，血清对AaPAL的反应性与牙周炎的存在相关，但与口腔中伴放线放线杆菌的检测无关。除了AaPAL之外，人血清和针对三种不同类型抗原（凝胶纯化的AaPAL、伴放线放线杆菌全细胞抗原和CsgA）的兔抗血清还识别口腔嗜血菌的推定PAL。结果表明，新的AaPAL是一种保守的细菌脂蛋白。它在体内表达并且具有强烈的免疫反应性。在AaPAL和口腔嗜血菌之间发现的抗原交叉反应可能会增强牙周炎中的局部和全身免疫炎症反应。
