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基于QTL剖析的玉米(Zea mays L.)株高和穗位高遗传基础研究

[Study on the genetic basis of plant height and ear height in maize (Zea mays L.) by QTL dissection].

作者信息

Lan Jin-Hao, Chu Dong

机构信息

Institute of Crop Breeding and Cultivation, Chinese Academy of Agricultural Sciences/Key Laboratory of Crop Genetics and Breeding, Ministry of Agriculture, Beijing 100081, China.

出版信息

Yi Chuan. 2005 Nov;27(6):925-34.

Abstract

One hundred and ninety-one F2 individuals derives from the cross, Mo17xHuangzao4, were genotyped by SSR and AFLP markers to construct the genetic linkage map, and 184 corresponding F2:3 families were phenotyped for maize (Zea mays L.) plant hright and ear height in Changping and Shunyi. A mixed linear model approach and its software were used to detect QTLs with main effect, QTLs involved in digenic interations and Q x E interations. In total, 7 QTLs of plant height, 18 pairs of digenic epistatic loci of plant height, 13 pairs of digenic epistatic loci of ear height were detected. It was found that 6 QTLs of plant height, 8 QTLs of ear height, and 4 pairs of digenic epistatic loci of plant height and ear height and a significant interaction with environments. Genetic compnents underlying these quantitative traits were analyzed, and the study showed that additive, dominance and epistaship between plant height and ear height, and the results described above were also discussed for their possible application in molecular breeding.

摘要

利用SSR和AFLP标记对191个源于Mo17×黄早四杂交组合的F2个体进行基因分型,构建遗传连锁图谱,并对184个相应的F2:3家系在昌平和平谷的玉米(Zea mays L.)株高和穗位高进行表型鉴定。采用混合线性模型方法及其软件检测主效应QTL、双基因互作QTL和Q×E互作。共检测到7个株高QTL、18对株高双基因上位性位点、13对穗位高双基因上位性位点。发现6个株高QTL、8个穗位高QTL以及4对株高和穗位高双基因上位性位点与环境存在显著互作。分析了这些数量性状的遗传成分,研究表明株高和穗位高之间存在加性、显性和上位性,并对上述结果在分子育种中的可能应用进行了讨论。

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