Zhu Weihong, Zhao Yikun, Liu Jingbao, Huang Lu, Lu Xiaomin, Kang Dingming
MOE Key Laboratory of Crop Heterosis and Utilization, College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China.
Cereal Crops Institute, Henan Academy of Agricultural Sciences, Henan Provincial Key Lab. of Maize Biology, Zhengzhou, 450002, China.
Cell Mol Biol (Noisy-le-grand). 2019 Feb 28;65(2):18-27.
In this study, the elite maize inbred line (Zheng683-1) was used as a recurrent parent and the four maize inbred lines (ZPH1388, ZPH5, Dong 237 and Chang 7-2) were used as donor parents. The four F1 hybrids were produced by crossing between them and were continued backcrossing to the recurrent parent to produce four BC3F1s. The BC3F1 were pollinated by selfing four generations to generate the recombinant inbred lines (BC3F5) that showed variation in plant height, ear height, leaf angle. There were 53 lines for Zheng683-1 x ZPH1388, 53 lines for Zheng683-1 x ZPH5, 48 lines for Zheng683-1 x Dong237 and 61 lines for Zheng683-1 x Chang 7-2. The four populations were genotyped by using SNP marker and identified the QTLs of targeted traits by using QTL IciMapping V4.1 software and stepwise regression analysis. The main results are as follows:1. 19 additive QTLs and 2 dominant QTLs about plant height were detected in four introgression lines, and 7, 4, 5, 5 QTLs related plant height in ZPH1388-IL, ZPH5-IL, Dong237-ILand Chang7-2-IL. Among the 21 plant height QTLs, the largest contribution to phenotypic variation was QTLqPHa12 from Dong237-IL population, which evaluated 43.44% of the phenotypic variation of plant height . Followed by qPHa3 from ZPH1388-IL, the phenotypic contribution rate was 20% 2. Sixteen QTLs related ear height were detected in the population of the 4 introgression lines, of which there were 15 additive and 1 dominant QTLs. In addition, 9, 3, and 4 QTLs were detected in ZPH1388-IL, ZPH5-IL and Dong237-IL respectively. Among the 16 ear height QTLs, the largest contribution to phenotypic variation was QTL (qEHa1) from the ZPH1388 IL, which recorded the phenotypic variation of ear height by 26.01%. Next the phenotype contribution rates were 22.05% and 21.46%, respectively for qEHa13 and qEHa15 from Dong237-IL. Fourteen QTLs related leaf angle was detected in the population of the introgression lines, of which there were 5 additive QTLs and 9 dominant QTLs. Moreover, 2, 9, and 3 QTLs were detected in ZPH1388-IL, ZPH5-IL, Dong237-IL, respectively. Among the 14 leaf angle QTLs, the largest contribution to phenotypic variation was QTL (qLAa4) from the Dong237-IL then qLAa3 from ZPH5-IL, detecting the phenotypic variation of leaf angle of 28.56% and 24.08%, respectively.The results showed that the QTL locus was characterized by uneven chromosome distribution. The chromosome 1, 2, 5, and 7 are the regions with the QTL concentrated distribution of plant type traits. The QTL of plant type also showed QTL concentrated distribution in some regions of chromosomes. For example, there are three QTLs about plant height and ear height in the region of chromosome bin1.02, three QTLs about plant and ear heights, as well as leaf angle in the region of chromosome bin 2.02. These areas are QTL enrichment region (or the hot spots) of the pleiotropic gene loci related the plant type and ear traits.
本研究以优良玉米自交系(郑683-1)为轮回亲本,以4个玉米自交系(ZPH1388、ZPH5、东237和昌7-2)为供体亲本。将它们杂交产生4个F1杂种,并继续与轮回亲本回交,获得4个BC3F1。对BC3F1进行4代自交授粉,产生了在株高、穗位高、叶夹角等方面表现出变异的重组自交系(BC3F5)。郑683-1×ZPH1388有53个株系,郑683-1×ZPH5有53个株系,郑683-1×东237有48个株系,郑683-1×昌7-2有61个株系。利用SNP标记对这4个群体进行基因分型,并使用QTL IciMapping V4.1软件和逐步回归分析鉴定目标性状的QTL。主要结果如下:1. 在4个导入系中检测到19个关于株高的加性QTL和2个显性QTL,ZPH1388-IL、ZPH5-IL、东237-IL和昌7-2-IL中与株高相关的QTL分别有7个、4个、5个、5个。在21个株高QTL中,对表型变异贡献最大的是东237-IL群体中的QTLqPHa12,其解释了株高表型变异的43.44%。其次是ZPH1388-IL中的qPHa3,表型贡献率为20%。2. 在4个导入系群体中检测到16个与穗位高相关的QTL,其中有15个加性QTL和1个显性QTL。此外,在ZPH1388-IL、ZPH5-IL和东237-IL中分别检测到9个、3个和4个QTL。在16个穗位高QTL中,对表型变异贡献最大的是ZPH1388 IL中的QTL(qEHa1),其记录了穗位高26.01%的表型变异。其次,东237-IL中的qEHa13和qEHa15的表型贡献率分别为22.05%和21.46%。在导入系群体中检测到14个与叶夹角相关的QTL,其中有5个加性QTL和9个显性QTL。此外,在ZPH1388-IL、ZPH5-IL、东237-IL中分别检测到2个、9个和3个QTL。在14个叶夹角QTL中,对表型变异贡献最大的是东237-IL中的QTL(qLAa4),其次是ZPH5-IL中的qLAa3,分别检测到叶夹角表型变异的28.56%和24.08%。结果表明,QTL位点在染色体上分布不均匀。第1、2、5和7号染色体是株型性状QTL集中分布的区域。株型QTL在染色体的一些区域也表现出QTL集中分布。例如,在染色体bin1.02区域有3个关于株高和穗位高的QTL,在染色体bin 2.02区域有3个关于株高、穗位高以及叶夹角的QTL。这些区域是与株型和穗部性状相关的多效基因位点的QTL富集区(或热点)。