牛疱疹病毒1型UL47蛋白在受感染细胞中的核质穿梭
Nucleocytoplasmic shuttling of bovine herpesvirus 1 UL47 protein in infected cells.
作者信息
Verhagen Janneke, Hutchinson Ian, Elliott Gillian
机构信息
Virus Assembly Group, Marie Curie Research Institute, Oxted, Surrey RH8 OTL, United Kingdom.
出版信息
J Virol. 2006 Jan;80(2):1059-63. doi: 10.1128/JVI.80.2.1059-1063.2006.
Abstract
Previous studies with transfected cells have shown that the herpes simplex virus type 1 (HSV-1) and bovine herpesvirus 1 (BHV-1) UL47 proteins shuttle between the nucleus and the cytoplasm. HSV-1 UL47 has also been shown to bind RNA. Here we examine the BHV-1 UL47 protein in infected cells using a green fluorescent protein-UL47-expressing virus. We show that UL47 is detected in the nucleus early in infection. We use fluorescence loss in photobleaching to show that nuclear UL47 undergoes rapid nucleocytoplasmic shuttling. Furthermore, we demonstrate that actinomycin D inhibits the reaccumulation of UL47 in the nuclei of infected cells. These results suggest that UL47 exhibits behavior similar to that of previously characterized RNA-transporting proteins.
摘要
先前对转染细胞的研究表明，单纯疱疹病毒1型（HSV-1）和牛疱疹病毒1型（BHV-1）的UL47蛋白在细胞核和细胞质之间穿梭。HSV-1 UL47也已被证明能结合RNA。在这里，我们使用表达绿色荧光蛋白-UL47的病毒来检测感染细胞中的BHV-1 UL47蛋白。我们发现，在感染早期，细胞核中可检测到UL47。我们利用光漂白荧光损失技术表明，细胞核中的UL47会进行快速的核质穿梭。此外，我们证明放线菌素D可抑制UL47在感染细胞核中的重新积累。这些结果表明，UL47的行为与先前已鉴定的RNA转运蛋白相似。
相似文献
1
Nucleocytoplasmic shuttling of bovine herpesvirus 1 UL47 protein in infected cells.
J Virol. 2006 Jan;80(2):1059-63. doi: 10.1128/JVI.80.2.1059-1063.2006.
2
RNA binding by the herpes simplex virus type 1 nucleocytoplasmic shuttling protein UL47 is mediated by an N-terminal arginine-rich domain that also functions as its nuclear localization signal.
J Virol. 2007 Mar;81(5):2283-96. doi: 10.1128/JVI.01677-06. Epub 2006 Dec 13.
3
Unidirectional complementation between glycoprotein B homologues of pseudorabies virus and bovine herpesvirus 1 is determined by the carboxy-terminal part of the molecule.
J Gen Virol. 1995 Jul;76 ( Pt 7):1623-35. doi: 10.1099/0022-1317-76-7-1623.
4
Glycoprotein D homologs in herpes simplex virus type 1, pseudorabies virus, and bovine herpes virus type 1 bind directly to human HveC(nectin-1) with different affinities.
Virology. 2001 Feb 1;280(1):7-18. doi: 10.1006/viro.2000.0747.
5
Characterization of a novel transferable CRM-1-independent nuclear export signal in a herpesvirus tegument protein that shuttles between the nucleus and cytoplasm.
J Virol. 2006 Oct;80(20):10021-35. doi: 10.1128/JVI.01322-06.
6
Homologous and heterologous interference requires bovine herpesvirus-1 glycoprotein D at the cell surface during virus entry.
J Gen Virol. 2000 Apr;81(Pt 4):1041-9. doi: 10.1099/0022-1317-81-4-1041.
7
Construction and characterization of a glycoprotein E gene-deleted bovine herpesvirus type 1 recombinant.
Am J Vet Res. 1999 Feb;60(2):227-32.
8
The most abundant protein in bovine herpes 1 virions is a homologue of herpes simplex virus type 1 UL47.
J Gen Virol. 1991 Dec;72 ( Pt 12):3077-84. doi: 10.1099/0022-1317-72-12-3077.
9
The effect of bovine herpesvirus type 1 glycoproteins gI and gIII on herpesvirus infections.
J Gen Virol. 1989 Jun;70 ( Pt 6):1561-9. doi: 10.1099/0022-1317-70-6-1561.
10
2,3,7,8-Tetrachlorodibenzo-p-dioxin modifies expression and nuclear/cytosolic localization of bovine herpesvirus 1 immediate-early protein (bICP0) during infection.
J Cell Biochem. 2010 Oct 1;111(2):333-42. doi: 10.1002/jcb.22700.
引用本文的文献
1
mRNA Splicing of and Secretion of Glycoprotein C (gC) Is Conserved among the .
Viruses. 2024 May 15;16(5):782. doi: 10.3390/v16050782.
2
VP8, the Major Tegument Protein of Bovine Herpesvirus-1, Is Partially Packaged during Early Tegument Formation in a VP22-Dependent Manner.
Viruses. 2021 Sep 17;13(9):1854. doi: 10.3390/v13091854.
3
The requirement of glycoprotein C (gC) for interindividual spread is a conserved function of gC for avian herpesviruses.
Sci Rep. 2021 Apr 8;11(1):7753. doi: 10.1038/s41598-021-87400-x.
4
Duck enteritis virus pUL47, as a late structural protein localized in the nucleus, mainly depends on residues 40 to 50 and 768 to 777 and inhibits IFN-β signalling by interacting with STAT1.
Vet Res. 2020 Nov 11;51(1):135. doi: 10.1186/s13567-020-00859-w.
5
Qualitative Differences in Capsidless L-Particles Released as a By-Product of Bovine Herpesvirus 1 and Herpes Simplex Virus 1 Infections.
J Virol. 2018 Oct 29;92(22). doi: 10.1128/JVI.01259-18. Print 2018 Nov 15.
6
Phosphorylation of Bovine Herpesvirus 1 VP8 Plays a Role in Viral DNA Encapsidation and Is Essential for Its Cytoplasmic Localization and Optimal Virion Incorporation.
J Virol. 2016 Apr 14;90(9):4427-4440. doi: 10.1128/JVI.00219-16. Print 2016 May.
7
Identification and functional characterization of the Varicella zoster virus ORF11 gene product.
Virology. 2011 Mar 30;412(1):156-66. doi: 10.1016/j.virol.2010.12.055. Epub 2011 Jan 26.
8
The capsid protein encoded by U(L)17 of herpes simplex virus 1 interacts with tegument protein VP13/14.
J Virol. 2010 Aug;84(15):7642-50. doi: 10.1128/JVI.00277-10. Epub 2010 May 26.
9
Herpes simplex virus proteins ICP27 and UL47 associate with polyadenylate-binding protein and control its subcellular distribution.
J Virol. 2010 Jan;84(1):270-9. doi: 10.1128/JVI.01740-09.
10
A UL47 gene deletion mutant of bovine herpesvirus type 1 exhibits impaired growth in cell culture and lack of virulence in cattle.
J Virol. 2010 Jan;84(1):445-58. doi: 10.1128/JVI.01544-09.
本文引用的文献
1
Nucleocytoplasmic shuttling revealed by FRAP and FLIP technologies.
Curr Opin Biotechnol. 2005 Feb;16(1):28-34. doi: 10.1016/j.copbio.2004.11.002.
2
Characterization of nuclear localization and export signals of the major tegument protein VP8 of bovine herpesvirus-1.
Virology. 2004 Jul 1;324(2):327-39. doi: 10.1016/j.virol.2004.03.042.
3
hnRNP proteins: localization and transport between the nucleus and the cytoplasm.
Trends Cell Biol. 1993 May;3(5):151-5. doi: 10.1016/0962-8924(93)90135-n.
4
Nuclear export of the APC tumour suppressor controls beta-catenin function in transcription.
EMBO J. 2003 Mar 3;22(5):1101-13. doi: 10.1093/emboj/cdg105.
5
Mapping of functional regions in the amino-terminal portion of the herpes simplex virus ICP27 regulatory protein: importance of the leucine-rich nuclear export signal and RGG Box RNA-binding domain.
J Virol. 2002 Dec;76(23):11866-79. doi: 10.1128/jvi.76.23.11866-11879.2002.
6
Identification and characterization of the pseudorabies virus tegument proteins UL46 and UL47: role for UL47 in virion morphogenesis in the cytoplasm.
J Virol. 2002 Sep;76(17):8820-33. doi: 10.1128/jvi.76.17.8820-8833.2002.
7
Of the three tegument proteins that package mRNA in herpes simplex virions, one (VP22) transports the mRNA to uninfected cells for expression prior to viral infection.
Proc Natl Acad Sci U S A. 2002 Jun 11;99(12):8318-23. doi: 10.1073/pnas.122231699.
8
Nup98 is a mobile nucleoporin with transcription-dependent dynamics.
Mol Biol Cell. 2002 Apr;13(4):1282-97. doi: 10.1091/mbc.01-11-0538.
9
Characterization of Marek's disease virus serotype 1 (MDV-1) deletion mutants that lack UL46 to UL49 genes: MDV-1 UL49, encoding VP22, is indispensable for virus growth.
J Virol. 2002 Feb;76(4):1959-70. doi: 10.1128/jvi.76.4.1959-1970.2002.
10
MAP kinase dynamics in yeast.
Biol Cell. 2001 Sep;93(1-2):63-70. doi: 10.1016/s0248-4900(01)01123-6.
Zotero 插件