Chase C C, Carter-Allen K, Letchworth G J
Department of Veterinary Science, University of Wisconsin-Madison 53706.
J Gen Virol. 1989 Jun;70 ( Pt 6):1561-9. doi: 10.1099/0022-1317-70-6-1561.
We expressed the bovine herpesvirus type 1 (BHV-1) glycoproteins, gI and gIII, in bovine cells using a bovine papillomavirus vector. The proteins expressed by these cells had the same Mr as the native BHV-1 proteins and monoclonal antibodies detected no differences in their antigenic structure. Cells expressing gI were infected with either BHV-1 or herpes simplex virus type 1 (HSV-1). The number of plaques in gI-expressing cells was similar to that seen with normal fibroblasts infected with BHV-1 or HSV-1. However, BHV-1 or HSV-1 plaques produced in gI-expressing cells were smaller and darker than those seen in normal fibroblasts indicating an interference with cell-to-cell transmission or cellular lysis. Virus growth curves and [35S]methionine labelling of BHV-1-infected gI-expressing cells showed no difference in virus production, virus protein synthesis or cellular protein shutdown when compared to BHV-1-infected normal cells. This led us to conclude that the gI protein may interfere with a cellular protein(s) responsible for the cytopathic effects of BHV-1 infection. Cells expressing gIII were fully susceptible to BHV-1 infection.
我们使用牛乳头瘤病毒载体在牛细胞中表达了牛疱疹病毒1型（BHV-1）糖蛋白gI和gIII。这些细胞表达的蛋白质与天然BHV-1蛋白质具有相同的相对分子质量，单克隆抗体检测到它们的抗原结构没有差异。用BHV-1或单纯疱疹病毒1型（HSV-1）感染表达gI的细胞。表达gI的细胞中噬斑数量与感染BHV-1或HSV-1的正常成纤维细胞中的噬斑数量相似。然而，在表达gI的细胞中产生的BHV-1或HSV-1噬斑比正常成纤维细胞中的噬斑更小、颜色更深，表明对细胞间传播或细胞裂解存在干扰。与感染BHV-1的正常细胞相比，BHV-1感染的表达gI的细胞的病毒生长曲线和[35S]甲硫氨酸标记显示在病毒产生、病毒蛋白合成或细胞蛋白关闭方面没有差异。这使我们得出结论，gI蛋白可能干扰负责BHV-1感染细胞病变效应的一种或多种细胞蛋白。表达gIII的细胞对BHV-1感染完全敏感。
