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1
Escherichia coli enterotoxin: stimulation of adenylate cyclase in broken-cell preparations.大肠杆菌肠毒素：破碎细胞制剂中腺苷酸环化酶的刺激作用
Infect Immun. 1975 Mar;11(3):429-35. doi: 10.1128/iai.11.3.429-435.1975.
2
Comparison of the action of Escherichia coli enterotoxin on the thymocyte adenylate cyclase-cyclic adenosine monophosphate system to that of cholera toxin and prostaglandin E1.大肠杆菌肠毒素对胸腺细胞腺苷酸环化酶 - 环磷酸腺苷系统的作用与霍乱毒素和前列腺素E1作用的比较。
Infect Immun. 1974 Sep;10(3):503-9. doi: 10.1128/iai.10.3.503-509.1974.
3
Action of Escherichia coli enterotoxin: adenylate cyclase behavior of intestinal epithelial cells in culture.大肠杆菌肠毒素的作用：培养的肠上皮细胞的腺苷酸环化酶活性
Infect Immun. 1974 Jun;9(6):1003-10. doi: 10.1128/iai.9.6.1003-1010.1974.
4
Comparison of the effects of enterotoxins of Shigella dysenteriae and Vibrio cholerae on the adenylate cyclase system of the rabbit intestine.
J Infect Dis. 1974 Oct;130(4):374-9. doi: 10.1093/infdis/130.4.374.
5
Stimulation of intestinal adenyl cyclase by Escherichia coli enterotoxin: comparison of strains from an infant and an adult with diarrhea.大肠杆菌肠毒素对肠道腺苷酸环化酶的刺激作用：来自一名腹泻婴儿和一名腹泻成人的菌株比较。
J Infect Dis. 1974 Jan;129(1):1-9. doi: 10.1093/infdis/129.1.1.
6
Stimulation of intestinal mucosal adenyl cyclase by cholera enterotoxin and prostaglandins.霍乱肠毒素和前列腺素对肠黏膜腺苷酸环化酶的刺激作用。
J Clin Invest. 1971 Jun;50(6):1218-30. doi: 10.1172/JCI106599.
7
Stimulation of adenyl cyclase by Escherichia coli enterotoxin.大肠杆菌肠毒素对腺苷酸环化酶的刺激作用。
Nat New Biol. 1972 Apr 5;236(66):137-8. doi: 10.1038/newbio236137a0.
8
Escherichia coli enterotoxin. Purification and partial characterization.大肠杆菌肠毒素。纯化及部分特性鉴定。
J Biol Chem. 1975 Nov 25;250(22):8712-9.
9
The effect of cholera toxin and heat labile and heat stable Escherichia coli enterotoxin on cyclic AMP concentrations in small intestinal mucosa of pig and rabbit.霍乱毒素、不耐热和耐热大肠杆菌肠毒素对猪和兔小肠黏膜中环磷酸腺苷浓度的影响。
Can J Comp Med. 1978 Jul;42(3):327-31.
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Glucose inhibition of adenylate cyclase in intact cells of Escherichia coli B.葡萄糖对大肠杆菌B完整细胞中腺苷酸环化酶的抑制作用。
Proc Natl Acad Sci U S A. 1974 Jun;71(6):2324-8. doi: 10.1073/pnas.71.6.2324.

引用本文的文献

1
Effect of lymphocytosis-promoting factor from Bordetella pertussis on cerebellar cyclic GMP levels.百日咳博德特氏菌促淋巴细胞增多因子对小脑环磷酸鸟苷水平的影响。
Infect Immun. 1982 Jun;36(3):958-61. doi: 10.1128/iai.36.3.958-961.1982.
2
Mechanism of action of choleragen and E. coli heat-labile enterotoxin: activation of adenylate cyclase by ADP-ribosylation.霍乱毒素和大肠杆菌不耐热肠毒素的作用机制：通过ADP-核糖基化激活腺苷酸环化酶。
Mol Cell Biochem. 1981 Jul 7;37(2):75-90. doi: 10.1007/BF02354931.
3
Functional significance of histologic alterations induced by Escherichia coli pig-specific, mouse-negative, heat-stable enterotoxin (STb).猪特异性、小鼠阴性、热稳定肠毒素（STb）诱导的组织学改变的功能意义。
Vet Res Commun. 1987;11(1):41-55. doi: 10.1007/BF00361325.
4
Assay of Escherichia coli enterotoxins by in vivo perfusion in the rat jejunum.通过大鼠空肠体内灌注法检测大肠杆菌肠毒素
Infect Immun. 1976 Oct;14(4):1004-10. doi: 10.1128/iai.14.4.1004-1010.1976.
5
Assay of Escherichia coli heat-labile enterotoxin with vero cells.用非洲绿猴肾细胞检测大肠杆菌不耐热肠毒素
Infect Immun. 1977 May;16(2):617-22. doi: 10.1128/iai.16.2.617-622.1977.
6
The effect of adsorbant and anti-inflammatory drugs on secretion in ligated segments of pig intestine infected with Escherichia coli.吸附剂和抗炎药物对感染大肠杆菌的猪肠结扎段分泌物的影响。
Can J Comp Med. 1978 Jul;42(3):260-8.
7
Activation of adenylate cyclase by heat-labile Escherichia coli enterotoxin. Evidence for ADP-ribosyltransferase activity similar to that of choleragen.不耐热的大肠杆菌肠毒素对腺苷酸环化酶的激活作用。存在与霍乱毒素类似的ADP-核糖基转移酶活性的证据。
J Clin Invest. 1978 Aug;62(2):281-5. doi: 10.1172/JCI109127.
8
Pathophysiological effects of Vibrio cholerae and enterotoxigenic Escherichia coli and their exotoxins on eucaryotic cells.霍乱弧菌和产肠毒素大肠杆菌及其外毒素对真核细胞的病理生理作用。
Microbiol Rev. 1978 Sep;42(3):592-613. doi: 10.1128/mr.42.3.592-613.1978.
9
Stimulation of cyclic AMP secretion in Vero cells by enterotoxins of Escherichia coli and Vibrio cholerae.大肠杆菌和霍乱弧菌的肠毒素对非洲绿猴肾细胞中环磷酸腺苷分泌的刺激作用。
Infect Immun. 1978 Aug;21(2):514-7. doi: 10.1128/iai.21.2.514-517.1978.

本文引用的文献

1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
The titration of cholera toxin and antitoxin in the rabbit ileal loop.兔肠袢中霍乱毒素与抗毒素的滴定
J Infect Dis. 1966 Dec;116(5):606-14. doi: 10.1093/infdis/116.5.606.
3
Cholera infection and toxin in the rabbit ileal loop.兔回肠袢中的霍乱感染与毒素
J Infect Dis. 1966 Apr;116(2):183-90. doi: 10.1093/infdis/116.2.183.
4
Enterotoxigenic Escherichia coli isolated from patients with severe cholera-like disease.从患有严重霍乱样疾病的患者中分离出的产肠毒素大肠杆菌。
J Infect Dis. 1971 Apr;123(4):378-85. doi: 10.1093/infdis/123.4.378.
5
Studies on enterotoxin from Escherichia coli associated with acute diarrhea in man.关于与人类急性腹泻相关的大肠杆菌肠毒素的研究。
J Lab Clin Med. 1971 Jul;78(1):81-7.
6
Comparative effects of enterotoxins from Escherichia coli and Vibrio cholerae on rabbit and swine small intestine.大肠杆菌和霍乱弧菌肠毒素对兔和猪小肠的比较作用。
Lab Invest. 1971 Aug;25(2):133-40.
7
The effect of cell-free fluids prepared from cultures of human and animal enteropathogenic strains of Escherichia coli on ligated intestinal segments of rabbits and pigs.从人和动物肠道致病性大肠杆菌培养物中制备的无细胞液对兔和猪结扎肠段的影响。
J Med Microbiol. 1970 Aug;3(3):403-9. doi: 10.1099/00222615-3-3-403.
8
The relationship between two apparently different enterotoxins produced by enteropathogenic strains of Escherichia coli of porcine origin.猪源致病性大肠杆菌产生的两种明显不同的肠毒素之间的关系。
J Med Microbiol. 1970 Aug;3(3):387-401. doi: 10.1099/00222615-3-3-387.
9
Response of the rabbit ileal loop to cell-free products from Escherichia coli enteropathogenic for swine.兔回肠袢对猪致病性大肠杆菌无细胞产物的反应。
J Infect Dis. 1970 Feb;121(2):182-7. doi: 10.1093/infdis/121.2.182.
10
A heat-labile enterotoxin from strains of Eschericha coli enteropathogenic for pigs.一种来自对猪具有致病性的大肠杆菌菌株的热不稳定肠毒素。
J Infect Dis. 1969 Oct;120(4):419-26. doi: 10.1093/infdis/120.4.419.

大肠杆菌肠毒素：破碎细胞制剂中腺苷酸环化酶的刺激作用

Escherichia coli enterotoxin: stimulation of adenylate cyclase in broken-cell preparations.

作者信息

Dorner F, Mayer P

出版信息

Infect Immun. 1975 Mar;11(3):429-35. doi: 10.1128/iai.11.3.429-435.1975.

DOI:10.1128/iai.11.3.429-435.1975
PMID:163792
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC415081/
Abstract

The enterotoxin from cell-free filtrates of the enteropathogenic Escherichia coli strain P-263 was found to stimulate adenylate cyclase activity in broken-cell preparations from myocardial tissue. Particulate and detergent-solubilized fractions from cat heart were incubated with enterotoxin and assayed for adenylate cyclase activity. Adenylate cyclase activity was stimulated by enterotoxin; the extent of stimulation was proportional to the concentration of enterotoxin. The data demonstrate that stimulation of enterotoxin-sensitive adenylate cyclase in this system provides a sensitive in vitro assay, either as an accurate measure of enterotoxin concentration or as an assay for antitoxic titers in sera. A parallel comparison showed that stimulation of fluid production in rabbit intestinal loops by enterotoxin was less sensitive.

摘要

研究发现，肠道致病性大肠杆菌P - 263菌株的无细胞滤液中的肠毒素，可刺激心肌组织破碎细胞制剂中的腺苷酸环化酶活性。将猫心脏的微粒体和经去污剂溶解的部分与肠毒素一起孵育，并测定腺苷酸环化酶活性。肠毒素可刺激腺苷酸环化酶活性；刺激程度与肠毒素浓度成正比。数据表明，该系统中对肠毒素敏感的腺苷酸环化酶的刺激提供了一种灵敏的体外检测方法，既可以作为肠毒素浓度的精确测量方法，也可以作为血清中抗毒素效价的检测方法。平行比较表明，肠毒素对兔肠袢液体分泌的刺激作用较不敏感。