Brockmann M-A, Ulmer S, Leppert J, Nadrowitz R, Wuestenberg R, Nolte I, Petersen D, Groden C, Giese A, Gottschalk S
Department of Neuroradiology, Faculty for Clinical Medicine Mannheim, University of Heidelberg, Theodor-Kutzer-Ufer 1-3, 68167 Mannheim, Germany.
Brain Res. 2006 Jan 12;1068(1):138-42. doi: 10.1016/j.brainres.2005.10.098. Epub 2005 Dec 27.
With increasing numbers of in vivo experiments in the field of neuroscience, the interest in methods for in vivo imaging of animal brains as small as those of mice has increased. Because highly specialized small bore scanners with high field strengths are not commonly available, clinical magnetic resonance imaging (cMRI) scanners have been used in the past to image rat and more recently also mouse brains in combination with specifically developed RF coils. These studies have demonstrated that imaging of small animal brains is feasible, and that tumor volumes measured by cMRI correlate well with histological tumor volume analysis. This protocol describes the cMRI settings at 1.5 T for imaging of mouse brain with resolutions up to 120 x 120 microm using an inexpensive, commercially available small loop surface coil. This allows easy establishment of a small animal MRI facility without the need for cost intensive dedicated small animal scanners or special custom made coils. In this study, we demonstrate high-resolution imaging of intracranial xenografts in a mouse glioma model and monitor the treatment effect of external field irradiation by cMRI.
随着神经科学领域体内实验数量的增加，对小鼠等小型动物大脑进行体内成像方法的兴趣也在增加。由于具有高场强的高度专业化小口径扫描仪并不常见，过去临床磁共振成像（cMRI）扫描仪已被用于对大鼠以及最近对小鼠大脑进行成像，并结合专门开发的射频线圈。这些研究表明，对小动物大脑进行成像可行，且通过cMRI测量的肿瘤体积与组织学肿瘤体积分析结果高度相关。本方案描述了在1.5 T场强下使用廉价的市售小环形表面线圈对小鼠大脑进行成像的cMRI设置，分辨率可达120×120微米。这使得无需昂贵的专用小动物扫描仪或特殊定制线圈就能轻松建立小动物MRI设施。在本研究中，我们展示了小鼠胶质瘤模型中颅内异种移植瘤的高分辨率成像，并通过cMRI监测外照射的治疗效果。
