Barsiene Janina, Schiedek Doris, Rybakovas Aleksandras, Syvokiene Janina, Kopecka Justyna, Förlin Lars
Institute of Ecology of Vilnius University, Akademijos 2, 08412 Vilnius, Lithuania.
Mar Pollut Bull. 2006;53(8-9):469-78. doi: 10.1016/j.marpolbul.2005.11.015. Epub 2005 Dec 27.
Frequency of micronuclei (MN) and other nuclear abnormalities (nuclear buds, bi-nucleated and fragmented-apoptotic cells) was analysed in gill cells of the blue mussel (Mytilus spp.) from selected coastal sites in the Baltic Sea--Kvädöfjärden (Sweden), Klaipeda-Būtinge zone (Lithuania), Gulf of Gdansk (Poland) and Wismar Bay (Germany). Samples were collected from 650 specimens during bi-annual sampling campaigns in 2001 and 2002. The lowest frequency of MN (0.37 MN/1000 cells) was found in blue mussels from the reference site (Kvädöfjärden). The highest MN values (up to 6.7 MN/1000 cells) were registered in blue mussels from the Gulf of Gdansk in autumn 2001 and 2002, and at Wismar Bay in spring 2001 (up to 5.06 MN/1000 cells). Gradients of MN incidences were observed when comparing the three studied locations in Wismar Bay, and at the Lithuanian coast before the crude oil spill in the Būtinge oil terminal. Moreover, significant seasonal and inter-location differences in the responses were documented (P<0.0001). Nuclear abnormalities were observed most frequently in blue mussels from the Gulf of Gdansk.
对来自波罗的海选定沿海地点——瑞典的克瓦德约尔登、立陶宛的克莱佩达-布廷格区、波兰的格但斯克湾和德国的维斯马湾的蓝贻贝（紫贻贝属）鳃细胞中的微核（MN）及其他核异常（核芽、双核和凋亡碎片细胞）频率进行了分析。在2001年和2002年每两年一次的采样活动中，从650个样本中采集了样本。在参考地点（克瓦德约尔登）的蓝贻贝中发现微核频率最低（0.37个微核/1000个细胞）。2001年和2002年秋季在格但斯克湾的蓝贻贝以及2照地点（克瓦德约尔登）的蓝贻贝中发现微核频率最低（0.37个微核/1000个细胞）。2001年和2002年秋季在格但斯克湾的蓝贻贝以及2001年春季在维斯马湾的蓝贻贝中记录到最高的微核值（高达6.7个微核/1000个细胞）（高达5.06个微核/1000个细胞）。在比较维斯马湾的三个研究地点以及布廷格油码头原油泄漏前立陶宛海岸的情况时，观察到微核发生率的梯度。此外，还记录到反应存在显著的季节和地点间差异（P<0.0001）。在格但斯克湾的蓝贻贝中观察到核异常最为频繁。
