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检测伊兹密尔湾(土耳其西部)一些水生生物中的微核频率,以监测污染情况。

Detecting micronuclei frequency in some aquatic organisms for monitoring pollution of Izmir Bay (Western Turkey).

机构信息

Faculty of Fisheries, Department of Hydrobiology, Ege University, Bornova, Izmir, Turkey.

出版信息

Environ Monit Assess. 2010 Jun;165(1-4):55-66. doi: 10.1007/s10661-009-0926-5. Epub 2009 May 15.

Abstract

Micronuclei tests is a system of mutagenicity testing used for determining the pollution and chemicals causing changes in DNA fragments such as micronuclei in the cytoplasm of interphase cells. Damage caused on the DNA by genotoxic pollutants is the first consequence occurring in the aquatic organisms. Thus, it was attempted to determine whether pollution affected the erythrocytes and gills of fish Gobius niger and haemolymph and gills of mussels Mytilus galloprovincialis living in Izmir Bay at the level of DNA by the means of micronuclei (MN) test. Organisms used in the MN test were collected from seven locations (Alsancak, Alaybey Shipyard, Karsiyaka, Bostanli, Göztepe, Konak and Pasaport) which are known as the most polluted part of inner Bay of Izmir (Western Coast of Turkey). According to the results of the present study, frequency of MN was found at high level in Alaybey Shipyard and Pasaport where wastes from existing dockyard contributed to high level of pollution. In conclusion, this study indicates that the micronuclei test gives sensitive results in monitoring the pollution, especially the pollution of harbor, and thus it might be used as standard method in regular monitoring of pollution of coastal ecosystem.

摘要

微核试验是一种用于检测诱变的系统,用于确定污染和化学物质是否导致间期细胞细胞质中的微核等 DNA 片段发生变化。遗传毒性污染物对 DNA 造成的损伤是水生生物中首先发生的后果。因此,人们试图通过微核(MN)试验确定污染是否在 DNA 水平上影响生活在伊兹密尔湾的黑鳍真鲨的红细胞和鳃以及贻贝的血淋巴和鳃。用于 MN 试验的生物体取自七个地点(Alsancak、Alaybey 造船厂、Karsiyaka、Bostanli、Göztepe、Konak 和 Pasaport),这些地点被认为是伊兹密尔内湾(土耳其西海岸)污染最严重的部分。根据本研究的结果,在 Alaybey 造船厂和 Pasaport 发现 MN 的频率很高,这是因为现有的造船厂的废物造成了高水平的污染。总之,这项研究表明,微核试验在监测污染方面提供了敏感的结果,特别是港口污染,因此它可能被用作沿海生态系统定期污染监测的标准方法。

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