Baker R E, Ruijter J M, Corner M A, Mud M T
Netherlands Institute for Brain Research, Amsterdam.
Brain Res Bull. 1992 Jun;28(6):975-8. doi: 10.1016/0361-9230(92)90220-r.
Chronic suppression of spontaneous bioelectric activity in spinal cord explants in the presence of tetrodotoxin (TTX) during network formation caused a large reduction in cell number (lowered DNA levels). The addition of gangliosides failed to protect against this cell loss. Conversely, the omission of galactose from the growth medium had no effect on DNA levels. It was concluded that the presence or absence of afferent selectivity is unlikely to require the survival of a regionally specific subpopulation of preferred dorsal root ganglion target cells. Neocortical explants also showed a large reduction in DNA levels following chronic TTX treatment, and morphometric analysis confirmed that neuronal survival was affected to the same degree. Chronic ganglioside supplementation failed to influence DNA and cell counts in either control or TTX-treated explants, but one of the added gangliosides (GD1a) stimulated extensive neuritic outgrowth in electrically silenced cultures. Particular ganglioside species, therefore, may exert a growth stimulating influence that can partially compensate for the absence of bioelectric self-stimulation during early development.
在网络形成过程中，在存在河豚毒素（TTX）的情况下对脊髓外植体的自发生物电活动进行慢性抑制，导致细胞数量大幅减少（DNA水平降低）。添加神经节苷脂未能防止这种细胞损失。相反，从生长培养基中去除半乳糖对DNA水平没有影响。得出的结论是，传入选择性的存在与否不太可能需要特定区域的首选背根神经节靶细胞亚群的存活。慢性TTX处理后，新皮质外植体的DNA水平也大幅降低，形态计量分析证实神经元存活受到同等程度的影响。慢性补充神经节苷脂未能影响对照或TTX处理的外植体中的DNA和细胞计数，但添加的一种神经节苷脂（GD1a）在电沉默培养物中刺激了广泛的神经突生长。因此，特定种类的神经节苷脂可能发挥生长刺激作用，可部分补偿早期发育过程中生物电自我刺激的缺失。
