Zinchenko Anatoly A, Yoshikawa Kenichi, Baigl Damien
Department of Physics, Graduate School of Science, Kyoto University, Kyoto 606-8502, Japan.
Phys Rev Lett. 2005 Nov 25;95(22):228101. doi: 10.1103/PhysRevLett.95.228101. Epub 2005 Nov 21.
We elaborated a versatile experimental model of chromatin which consists of a single chain of long duplex DNA that interacts with well-defined cationic nanoparticles of various sizes. We found that the DNA compaction by nanoparticles is stepwise and progressive at the single-chain level. It is controlled by the ability of DNA to wrap nanoparticles, which is more efficient for larger particles and, similar to DNA-histone interaction, is optimal at a physiological salt concentration.
我们构建了一种通用的染色质实验模型，该模型由一条长双链DNA单链组成，其与各种大小明确的阳离子纳米颗粒相互作用。我们发现，在单链水平上，纳米颗粒对DNA的压缩是逐步且渐进的。它受DNA包裹纳米颗粒能力的控制，对于较大颗粒这种能力更强，并且与DNA-组蛋白相互作用类似在生理盐浓度下最为理想。
