Paine M A, Scioscia Marco, Gumaa Khalid A, Rodeck C H, Rademacher T W
Department of Molecular Pathology and Immunology, Middlesex Hospital, 1 Mortimer Street, University College London, London W1T 8AA, UK.
J Reprod Immunol. 2006 Apr;69(2):165-79. doi: 10.1016/j.jri.2005.09.008. Epub 2005 Dec 27.
Abnormal secretion of P-type inositol phosphoglycans (IPG-P) has been described in maternal urine of pre-eclamptic women. The aim of this study was to determine the origin of production of IPG-P. We examined the IPG-P content of maternal and fetal serum, maternal urine and amniotic fluid in both normal pregnancy and pre-eclampsia.
Established extraction and bioactivity assay techniques were used to compare total IPG-P levels in serum samples, and a polyclonal-antibody-based ELISA to assay the amniotic fluid and urine samples in matched pairs of women.
Eleven women with pre-eclampsia requiring caesarean section (subjects), 11 pregnant women requiring elective caesarean section for reasons other than pre-eclampsia (controls).
Our data confirm the abnormal level of IPG-P in maternal urine during pre-eclampsia. Moreover, IPG-P levels were higher in umbilical sera than in maternal sera samples. Amniotic fluid as well as urine ELISA results were significantly higher in the pre-eclamptic group compared with normal controls. Total IPG-P bioactivity in serum did not vary between serum compartments in normal pregnancy. Uterine vein IPG-P levels were lower in pre-eclampsia when compared with normal pregnancy. A possible correlation was observed between urine and amniotic fluid levels in normal women. No correlation was observed between measured blood levels and those in urine and amniotic fluid.
It is hypothesized that steady state equilibrium of IPG-P in serum in normal pregnancy is disrupted in pre-eclampsia. Additionally, an abnormal IPG-P sub-fraction, detectable in urine and amniotic fluid, may be present and involved in the pathophysiology of the syndrome, although sites of production of this abnormal form remain unclear.
子痫前期孕妇的母体尿液中已发现P型肌醇磷酸聚糖（IPG-P）分泌异常。本研究的目的是确定IPG-P的产生来源。我们检测了正常妊娠和子痫前期孕妇的母体血清、胎儿血清、母体尿液和羊水的IPG-P含量。
采用既定的提取和生物活性测定技术比较血清样本中的总IPG-P水平，并使用基于多克隆抗体的ELISA法检测配对女性的羊水和尿液样本。
11例需要剖宫产的子痫前期女性（研究对象），11例因子痫前期以外的原因需要择期剖宫产的孕妇（对照组）。
我们的数据证实了子痫前期孕妇母体尿液中IPG-P水平异常。此外，脐血血清中的IPG-P水平高于母体血清样本。与正常对照组相比，子痫前期组的羊水和尿液ELISA结果显著更高。正常妊娠时血清中总IPG-P生物活性在不同血清成分之间无差异。与正常妊娠相比，子痫前期时子宫静脉IPG-P水平较低。在正常女性中，尿液和羊水水平之间观察到可能的相关性。在测量的血液水平与尿液和羊水水平之间未观察到相关性。
据推测，子痫前期会破坏正常妊娠时血清中IPG-P的稳态平衡。此外，在尿液和羊水中可检测到的异常IPG-P亚组分可能存在并参与该综合征的病理生理过程，尽管这种异常形式的产生部位尚不清楚。
