Arikan Cigdem, Berdeli Afig, Ozgenc Funda, Tumgor Gokhan, Yagci Rasit V, Aydogdu Sema
Pediatric Gastroenterology, Ege University School of Medicine, Izmir, Turkey.
J Pediatr Gastroenterol Nutr. 2006 Jan;42(1):77-82. doi: 10.1097/01.mpg.0000192247.55583.fa.
Macrophage migration inhibitory factor (MIF) is a pleiotrophic lymphocyte and macrophage cytokine; it is likely to play an important role in innate immunity. Its expression was increased in several inflammatory diseases, and MIF gene polymorphisms have an effect on disease outcome and response to glucocorticoid treatment.
To investigate the role of the 173G/C polymorphism of the MIF gene for susceptibility to biliary atresia (BA).
Between February 2002 and November 2004, 18 patients (mean age 1 +/- 0.4 years) diagnosed as having BA were studied. After informed consent, blood was collected, and DNA was obtained. MIF 173C/G polymorphism was detected using the polymerase chain reaction-restriction fragment length polymorphism based method. BA patients were compared with a group of chronic liver disease patients (CLD) (n = 36) and a group of unrelated healthy controls (n = 103).
MIF-173C allele frequency was significantly higher than both the CLD and healthy control groups (P = 0.03, odds ratio [OR] 4.4, 95% confidence interval [CI] 1.3-15.1; P = 0.000, OR 4.1, 95% CI 2.3-7.6, respectively). Univariate analysis showed that MIF-173G/C polymorphism was significantly associated with BA (for GC genotype, OR = 6, 95 % CI 2.8-11.5, P = 0.000). There was no significant correlation between pediatric end stage liver disease score and MIF genotypes both in BA and CLD groups.
Our results suggest that the -173C allele of the MIF gene might be associated with the susceptibility to BA.
巨噬细胞移动抑制因子（MIF）是一种具有多种功能的淋巴细胞和巨噬细胞细胞因子；它可能在先天免疫中发挥重要作用。其表达在几种炎症性疾病中增加，并且MIF基因多态性对疾病结局和糖皮质激素治疗反应有影响。
研究MIF基因173G/C多态性在胆道闭锁（BA）易感性中的作用。
2002年2月至2004年11月，对18例诊断为BA的患者（平均年龄1±0.4岁）进行研究。在获得知情同意后，采集血液并提取DNA。采用基于聚合酶链反应-限制性片段长度多态性的方法检测MIF 173C/G多态性。将BA患者与一组慢性肝病患者（CLD）（n = 36）和一组无关健康对照者（n = 103）进行比较。
MIF-173C等位基因频率显著高于CLD组和健康对照组（分别为P = 0.03，优势比[OR] 4.4，95%置信区间[CI] 1.3 - 15.1；P = 0.000，OR 4.1，95% CI 2.3 - 7.6）。单因素分析显示MIF-173G/C多态性与BA显著相关（对于GC基因型，OR = 6，95% CI 2.8 - 11.5，P = 0.000）。在BA组和CLD组中，儿童终末期肝病评分与MIF基因型之间均无显著相关性。
我们的结果表明，MIF基因的-173C等位基因可能与BA易感性相关。
