Wu N Z, Klitzman B, Dodge R, Dewhirst M W
Department of Radiation Oncology, Duke University Medical Center, Durham, North Carolina 27710.
Cancer Res. 1992 Aug 1;52(15):4265-8.
Leukocyte-endothelium interaction in vivo consists of the rolling of leukocytes along the vascular wall and, under certain conditions, their adherence to endothelial cells. In a rat tumor microcirculation model (mammary adenocarcinoma implanted in rat skinfold window chamber), we demonstrated that this interaction, measured as flux of rolling leukocytes and density of adhering leukocytes, was significantly reduced in tumor microvessels compared to normal microvessels, both under control conditions and during inflammation induced by N-formylmethionylleucylphenylalanine (1 microM), bacterial lipopolysaccharide (1 microgram/ml), or tumor necrosis factor alpha (500 units/ml). We also measured the blood flow shear rate in the tumor and normal microvessels and found that the difference in shear rate between the two types of microvessels could not account for the differences in leukocyte-endothelium interaction. The diminished leukocyte-endothelium interaction in tumors under various stimulated conditions suggests that a number of adhesion molecules may not be expressed properly on tumor endothelial cells.
体内白细胞与内皮细胞的相互作用包括白细胞沿血管壁滚动，在某些情况下，还包括它们与内皮细胞的黏附。在大鼠肿瘤微循环模型（将乳腺腺癌植入大鼠皮肤褶窗小室）中，我们证明，在对照条件下以及在由N-甲酰甲硫氨酰亮氨酰苯丙氨酸（1微摩尔）、细菌脂多糖（1微克/毫升）或肿瘤坏死因子α（500单位/毫升）诱导的炎症期间，与正常微血管相比，肿瘤微血管中这种以滚动白细胞通量和黏附白细胞密度衡量的相互作用显著降低。我们还测量了肿瘤和正常微血管中的血流剪切率，发现两种类型微血管之间的剪切率差异不能解释白细胞与内皮细胞相互作用的差异。在各种刺激条件下肿瘤中白细胞与内皮细胞相互作用的减弱表明，一些黏附分子可能在肿瘤内皮细胞上不能正常表达。
