Kvellestad Agnar, Falk Knut, Nygaard Solveig M R, Flesja Kjell, Holm Jan Arne
Department of Basal Medicine and Aquatic Sciences, Norwegian School of Veterinary Science, PO Box 8146 Dep., 0033 Oslo, Norway.
Dis Aquat Organ. 2005 Nov 9;67(1-2):47-54. doi: 10.3354/dao067047.
Proliferative gill inflammation (PGI) causes significant losses in farmed Atlantic salmon Salmo salar L. in Norway, especially during the first months following seawater transfer. The aetiology is apparently multifactorial, including infection with chlamydia-like bacteria and Atlantic salmon paramyxovirus (ASPV). In the present study, gills from diseased fish from 3 farms on the western coast of Norway were sampled. The pathological changes were briefly described and the aetiological significance of ASPV studied by immunofluorescent staining of cryosections and by immunohistochemistry on sections of formalin-fixed and paraffin-embedded tissue. The pathological changes were macroscopically characterized by palour of the gills, and histologically by inflammation, circulatory disturbances, cell death and epithelial cell proliferation. ASPV was demonstrated in fish from all farms studied, as immunostaining consistent with ASPV was obtained in lamellar epithelial and endothelial cells of pathologically altered tissues. It is concluded that ASPV is at least a contributing cause of PGI. As far as we know, this is the first demonstration of fish disease related to infection with a paramyxovirus.
增殖性鳃炎（PGI）给挪威养殖的大西洋鲑（Salmo salar L.）造成了重大损失，尤其是在转入海水后的头几个月。其病因显然是多因素的，包括感染衣原体样细菌和大西洋鲑副粘病毒（ASPV）。在本研究中，对挪威西海岸3个养殖场的患病鱼的鳃进行了采样。简要描述了病理变化，并通过冷冻切片的免疫荧光染色以及对福尔马林固定石蜡包埋组织切片进行免疫组织化学研究了ASPV的病因学意义。病理变化在宏观上表现为鳃色变淡，组织学上表现为炎症、循环障碍、细胞死亡和上皮细胞增殖。在所研究的所有养殖场的鱼中均检测到了ASPV，因为在病理改变组织的片状上皮细胞和内皮细胞中获得了与ASPV一致的免疫染色。得出的结论是，ASPV至少是PGI的一个促成因素。据我们所知，这是首次证明与副粘病毒感染相关的鱼类疾病。
