Simpson C F, Hanley J E, Gaskin J M
J Infect Dis. 1975 Apr;131(4):390-6. doi: 10.1093/infdis/131.4.390.
Herpesvirus was detected by electron microscopy in hepatocytes of psittacine birds that died at a Florida aviary. The virus was identified in hepatocytes of chick embryos and budgerigars that were given injections of liver suspensions from naturally infected psittacines. Infected hepatocytes had prominent intranuclear inclusions that contained naked nucleocapsids. Both naked and enveloped virions were seen in the cytoplasm of hepatocytes, and enveloped nucleocapsids occasionally were seen in the perinuclear cisternae and extracellularly. The experimental and spontaneous disease mimicked a condition in parrots described previously by Pacheco. The viral agent was classified as Herpesvirus on the bases of the character of the intranuclear inclusions, the size and conformation of the agent as determined by electron microscopy and filtration, and the sensitivity of the agent to ether.
在佛罗里达州一家鸟类饲养场死亡的鹦鹉的肝细胞中，通过电子显微镜检测到疱疹病毒。从自然感染鹦鹉的肝脏悬液注射到鸡胚和虎皮鹦鹉体内后，在它们的肝细胞中鉴定出了该病毒。受感染的肝细胞有明显的核内包涵体，其中含有裸露的核衣壳。在肝细胞的细胞质中可见裸露和有包膜的病毒粒子，偶尔在核周池和细胞外可见有包膜的核衣壳。实验性和自发性疾病类似于先前帕切科描述的鹦鹉疾病。根据核内包涵体的特征、通过电子显微镜和过滤确定的病原体大小和形态以及病原体对乙醚的敏感性，将该病毒病原体归类为疱疹病毒。
