使用不对称流场-流分级分离-多角度光散射技术对大肠杆菌中绿色荧光蛋白包涵体进行尺寸表征。
Size characterization of green fluorescent protein inclusion bodies in E. coli using asymmetrical flow field-flow fractionation-multi-angle light scattering.
作者信息
Luo Jian, Leeman Mats, Ballagi Andras, Elfwing Anders, Su Zhiguo, Janson Jan-Christer, Wahlund Karl-Gustav
机构信息
National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, P.O. Box 353, Beijing 100080, PR China.
出版信息
J Chromatogr A. 2006 Jul 7;1120(1-2):158-64. doi: 10.1016/j.chroma.2005.11.048. Epub 2006 Jan 18.
The goal of this study was to investigate the applicability of asymmetrical flow field-flow fractionation-multi-angle light scattering (AsFlFFF-MALS) for size analysis of green fluorescent protein inclusion bodies (GFPIBs). The size distributions of GFPIBs prepared by various culture conditions were determined. For GFPIBs prepared at 37 degrees C the peak maximum hydrodynamic diameter (d(H)) first increased and then decreased with the increase of the induction times in the presence of 0.1 and 2 mM isopropyl-beta-D-thiogalactoside (IPTG). For GFPIBs prepared at 30 degrees C the peak maximum d(H) was constant at about 700 nm irrespectively of the induction times and IPTG concentrations.
本研究的目的是探讨不对称流场流分馏-多角度光散射（AsFlFFF-MALS）在绿色荧光蛋白包涵体（GFPIBs）尺寸分析中的适用性。测定了在各种培养条件下制备的GFPIBs的尺寸分布。对于在37℃制备的GFPIBs，在存在0.1和2 mM异丙基-β-D-硫代半乳糖苷（IPTG）的情况下，随着诱导时间的增加，最大峰值流体动力学直径（d(H)）先增加后减小。对于在30℃制备的GFPIBs，无论诱导时间和IPTG浓度如何，最大峰值d(H)在约700 nm处保持恒定。
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