Zoppa Mariella, Gallo Lorena, Zacchello Franco, Giordano Giuseppe
Department of Pediatrics, University of Padua, 35128 Padua, Italy.
J Chromatogr B Analyt Technol Biomed Life Sci. 2006 Feb 2;831(1-2):267-73. doi: 10.1016/j.jchromb.2005.12.015. Epub 2006 Jan 4.
In our study we have developed an HPLC-ESI-MS/MS method for qualitative and quantitative analysis of underivatized amino acids on dry blood spots. The sensitive and specific instrumental performances permitted the chromatographic separation of 40 amino acids and their isomers within 10 min. The method has been set up for cases of suspected metabolic diseases revealed by newborn screening. What is new is that it is applied on the same blood spots used for newborn screening, instead of plasma, in order to avoid involvement of doctors, increased anxiety for parents, stress for patients for plasma collection, long time of waiting and further costs for analysis.
在我们的研究中，我们开发了一种HPLC-ESI-MS/MS方法，用于对干血斑上未衍生化的氨基酸进行定性和定量分析。灵敏且特异的仪器性能使得40种氨基酸及其异构体在10分钟内实现色谱分离。该方法已针对新生儿筛查发现的疑似代谢疾病病例建立。新的地方在于，它应用于用于新生儿筛查的同一血斑，而非血浆，以避免医生的参与、增加家长的焦虑、患者采集血浆时的压力、长时间等待以及进一步的分析成本。
