Yang Ruiyue, Dong Jun, Guo Hanbang, Li Hongxia, Wang Shu, Zhao Haijian, Zhou Weiyan, Yu Songlin, Wang Mo, Chen Wenxiang
The Key Laboratory of Geriatrics, Beijing Hospital and Beijing Institute of Geriatrics, Ministry of Health, Beijing, China.
PLoS One. 2013 Dec 5;8(12):e81144. doi: 10.1371/journal.pone.0081144. eCollection 2013.
Serum branched-chain and aromatic amino acids (BCAAs and AAAs) have emerged as predictors for the future development of diabetes and may aid in diabetes risk assessment. However, the current methods for the analysis of such amino acids in biological samples are time consuming.
An isotope dilution liquid chromatography tandem mass spectrometry (ID-LC/MS/MS) method for serum BCAAs and AAAs was developed. The serum was mixed with isotope-labeled BCAA and AAA internal standards and the amino acids were extracted with acetonitrile, followed by analysis using LC/MS/MS. The LC separation was performed on a reversed-phase C18 column, and the MS/MS detection was performed via the positive electronic spray ionization in multiple reaction monitoring mode.
Specific analysis of the amino acids was achieved within 2 min. Intra-run and total CVs for the amino acids were less than 2% and 4%, respectively, and the analytical recoveries ranged from 99.6 to 103.6%.
A rapid and precise method for the measurement of serum BCAAs and AAAs was developed and may serve as a quick tool for screening serum BCAAs and AAAs in studies assessing diabetes risk.
血清支链氨基酸和芳香族氨基酸（BCAAs和AAAs）已成为糖尿病未来发展的预测指标，可能有助于糖尿病风险评估。然而，目前分析生物样品中此类氨基酸的方法耗时较长。
建立了一种用于血清BCAAs和AAAs的同位素稀释液相色谱串联质谱（ID-LC/MS/MS）方法。将血清与同位素标记的BCAA和AAA内标混合，用乙腈提取氨基酸，然后用LC/MS/MS进行分析。LC分离在反相C18柱上进行，MS/MS检测通过正电喷雾电离在多反应监测模式下进行。
在2分钟内实现了对氨基酸的特异性分析。氨基酸的批内和总变异系数分别小于2%和4%，分析回收率在99.6%至103.（此处原文可能有误，推测应为103.6%）6%之间。
开发了一种快速、精确测量血清BCAAs和AAAs的方法，可作为在评估糖尿病风险的研究中筛查血清BCAAs和AAAs的快速工具。
