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[从大鼠胰腺中分离胰岛并测定其中的蛋白水解活性]

[Isolation of the islands of Langerhans from rat pancreas and determination of proteolytic activity in them].

作者信息

Pigareva M I, Beliaeva N F, Starosel'tseva L K

出版信息

Vopr Med Khim. 1976 Jan-Feb;22(1):137-40.

PMID:16389
Abstract

A modified method for isolation of Langerhans islands from rat pancreas is described. The islands obtained were free from acinous tissue as shown by means of an amylase test and by microscopy. In homogenates of the islands proteolytic activities were observed (pH optimum at 5.3--5.5 and 6.9--7.2).

摘要

本文描述了一种从大鼠胰腺中分离胰岛的改良方法。通过淀粉酶测试和显微镜观察表明,所获得的胰岛不含腺泡组织。在胰岛匀浆中观察到蛋白水解活性(最适pH值为5.3 - 5.5和6.9 - 7.2)。

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