用于酶法测定葡萄酒中乙醇含量的顺序注射系统。

Sequential injection system for the enzymatic determination of ethanol in wine.

作者信息

Páscoa Ricardo N M J, Vidigal Susana S M P, Tóth Ildikó V, Rangel António O S S

机构信息

Escola Superior de Biotecnologia, Universidade Católica Portuguesa, Rua Dr. António Bernardino de Almeida, 4200-072 Porto, Portugal.

出版信息

J Agric Food Chem. 2006 Jan 11;54(1):19-23. doi: 10.1021/jf051787l.

DOI:10.1021/jf051787l

PMID:16390171

Abstract

A sequential injection system was developed for the enzymatic determination of ethanol in wine. The spectrophotometric determination is based on the enzymatic reaction catalyzed by alcohol dehydrogenase in the presence of NAD+. The system was applied to the determination of ethanol in a range of 0.008-0.024% (v/v) with good repeatability; RSD(n=10) < 2.3%. The results obtained with the developed system showed good agreement with those obtained by using the reference method. The determination rate was 25 h(-1); 1 micromol of NAD+, 1.1 units of enzyme, and 50 microL of sample were consumed per determination; and the waste produced was 2.2 mL per assay.

摘要

开发了一种用于酶法测定葡萄酒中乙醇的顺序注射系统。分光光度法测定基于在NAD⁺存在下由乙醇脱氢酶催化的酶促反应。该系统用于测定0.008 - 0.024%（v/v）范围内的乙醇，具有良好的重复性；相对标准偏差（n = 10）< 2.3%。所开发系统获得的结果与使用参考方法获得的结果显示出良好的一致性。测定速率为25 h⁻¹；每次测定消耗1 μmol的NAD⁺、1.1个酶单位和50 μL样品；每次测定产生的废液为2.2 mL。