使用带分光光度检测的顺序注射系统对葡萄酒和啤酒中的甘油进行动力学酶法测定。

Kinetic enzymatic determination of glycerol in wine and beer using a sequential injection system with spectrophotometric detection.

作者信息

Oliveira Hugo M, Segundo Marcela A, Lima José L F C, Grassi Viviane, Zagatto Elias A G

机构信息

REQUIMTE, Serviço de Química-Física, Faculdade de Farmacia, Universidade do Porto, Rua Aníbal Cunha 164, 4099-030 Porto, Portugal.

出版信息

J Agric Food Chem. 2006 Jun 14;54(12):4136-40. doi: 10.1021/jf060229j.

DOI:10.1021/jf060229j

PMID:16756338

Abstract

A sequential injection system for the automatic determination of glycerol in wine and beer was developed. The method is based on the rate of formation of NADH from the reaction of glycerol and NAD+ catalyzed by the enzyme glycerol dehydrogenase in solution. The determination of glycerol was performed between 0.3 and 3.0 mmol L(-1) (0.028 and 0.276 g L(-1)), and good repeatability was attained (rsd < 3.6%, n = 5) for all samples tested. The determination rate was 54 h(-1), the reagent consumption was only 0.75 micromol of NAD+ and 5.4 ng of enzyme per assay, and the waste production was 2.12 mL per assay. Results obtained for samples were in agreement with those obtained with the batch enzymatic method.

摘要

开发了一种用于自动测定葡萄酒和啤酒中甘油的顺序注射系统。该方法基于溶液中甘油脱氢酶催化甘油与NAD⁺反应生成NADH的速率。甘油的测定范围为0.3至3.0 mmol L⁻¹（0.028至0.276 g L⁻¹），对所有测试样品均具有良好的重复性（相对标准偏差<3.6%，n = 5）。测定速率为54 h⁻¹，每次测定的试剂消耗量仅为0.75 μmol的NAD⁺和5.4 ng的酶，每次测定的废物产量为2.12 mL。样品的测定结果与批量酶法获得的结果一致。