基质金属蛋白酶-13（MMP-13）在口腔癌多阶段发生过程中的表达增加以及表没食子儿没食子酸酯（EGCG）抑制MMP-13的表达

Increase of MMP-13 expression in multi-stage oral carcinogenesis and epigallocatechin-3-gallate suppress MMP-13 expression.

作者信息

Chiang W-C, Wong Y-K, Lin S-C, Chang K-W, Liu C-J

机构信息

The School of Dentistry, National Yang-Ming University Taipei, Taiwan.

出版信息

Oral Dis. 2006 Jan;12(1):27-33. doi: 10.1111/j.1601-0825.2005.01151.x.

DOI:10.1111/j.1601-0825.2005.01151.x

PMID:16390465

Abstract

BACKGROUND

Matrix metalloproteinases (MMPs) play pivotal roles in tumor progression. MMP-13 (collagenase-3) digests collagen and other extracellular components.

MATERIALS AND METHODS

Reverse transcriptase-polymerase chain reaction (RT-PCR), immunohistochemistry and zymograph were used to study the roles of MMP-13 during the neoplastic process of oral squamous cell carcinoma (OSCC).

RESULTS

Increase of MMP-13 mRNA and protein expression in OSCC cell lines relative to cultivated normal oral keratinocytes was found. MMP-13 mRNA expression in OSCC was significantly higher than in non-cancerous match tissue (NCMT) in 36 tissue pairs. Esophageal squamous cell carcinoma also exhibited high MMP-13 mRNA expression. The percentage of OSCC exhibiting strong MMP-13 immunoreactivity was significantly higher than pre-invasive lesion and NCMT. Treatment with >5 microm epigallocatechin-3-gallate (EGCG) to OEC-M1 cells suppressed the expression and activity of MMP-13.

CONCLUSION

MMP-13 could be a potential tumor marker for OSCC. The effects of EGCG in tumor inhibition may act partially through the modulation of MMP-13.

摘要

背景

基质金属蛋白酶（MMPs）在肿瘤进展中起关键作用。MMP - 13（胶原酶 - 3）可消化胶原蛋白和其他细胞外成分。

材料与方法

采用逆转录聚合酶链反应（RT - PCR）、免疫组织化学和酶谱分析法研究MMP - 13在口腔鳞状细胞癌（OSCC）肿瘤形成过程中的作用。

结果

相对于培养的正常口腔角质形成细胞，OSCC细胞系中MMP - 13 mRNA和蛋白表达增加。在36对组织中，OSCC的MMP - 13 mRNA表达显著高于癌旁匹配组织（NCMT）。食管鳞状细胞癌也表现出较高的MMP - 13 mRNA表达。显示强MMP - 13免疫反应性的OSCC百分比显著高于癌前病变和NCMT。用>5微米表没食子儿茶素 - 3 - 没食子酸酯（EGCG）处理OEC - M1细胞可抑制MMP - 13的表达和活性。