Vincent-Chong Vui King, Salahshourifar Iman, Karen-Ng Lee Peng, Siow Ming Yhong, Kallarakkal Thomas George, Ramanathan Anand, Yang Yi-Hsin, Khor Goot Heah, Rahman Zainal Ariff Abdul, Ismail Siti Mazlipah, Prepageran Narayanan, Mustafa Wan Mahadzir Wan, Abraham Mannil Thomas, Tay Keng Kiong, Cheong Sok Ching, Zain Rosnah Binti
Oral Cancer Research and Coordinating Centre, Faculty of Dentistry, University of Malaya, 50603 Kuala Lumpur, Malaysia ; Department of Oro-Maxillofacial Surgical and Medical Sciences, Faculty of Dentistry, University of Malaya, 50603 Kuala Lumpur, Malaysia.
Oral Cancer Research and Coordinating Centre, Faculty of Dentistry, University of Malaya, 50603 Kuala Lumpur, Malaysia.
ScientificWorldJournal. 2014;2014:897523. doi: 10.1155/2014/897523. Epub 2014 Oct 23.
Matrix metalloproteinase 13 (MMP13) plays a central role in the MMP activation cascade that enables degradation of the extracellular matrix and basement membranes, and it is identified as a potential driver in oral carcinogenesis. Therefore, this study aims to determine the copy number, mRNA, and protein expression of MMP13 in oral squamous cell carcinoma (OSCC) and to associate these expressions with clinicopathological parameters. Copy number, mRNA, and protein expression analysis of MMP13 were determined using real-time quantitative PCR and immunohistochemistry methods in OSCC samples. The correlations between MMP13 expressions and clinicopathological parameters were evaluated, and the significance of MMP13 as a prognostic factor was determined. Despite discrepancies between gene amplification and mRNA and protein overexpression rates, OSCC cases showed high amplification of MMP13 and overexpression of MMP13 at both mRNA and protein levels. High level of MMP13 protein expression showed a significant correlation with lymph node metastasis (P = 0.011) and tumor staging (P = 0.002). Multivariate Cox regression model analysis revealed that high level of mRNA and protein expression of MMP13 were significantly associated with poor prognosis (P < 0.050). Taken together, these observations indicate that the MMP13 protein overexpression could be considered as a prognostic marker of OSCC.
基质金属蛋白酶13（MMP13）在MMP激活级联反应中起核心作用，该级联反应能够降解细胞外基质和基底膜，并且它被确定为口腔癌发生的潜在驱动因素。因此，本研究旨在确定口腔鳞状细胞癌（OSCC）中MMP13的拷贝数、mRNA和蛋白表达，并将这些表达与临床病理参数相关联。使用实时定量PCR和免疫组织化学方法在OSCC样本中测定MMP13的拷贝数、mRNA和蛋白表达分析。评估MMP13表达与临床病理参数之间的相关性，并确定MMP13作为预后因素的意义。尽管基因扩增与mRNA和蛋白过表达率之间存在差异，但OSCC病例显示出MMP13的高扩增以及mRNA和蛋白水平上MMP13的过表达。高水平的MMP13蛋白表达与淋巴结转移（P = 0.011）和肿瘤分期（P = 0.002）显示出显著相关性。多变量Cox回归模型分析显示，MMP13的高水平mRNA和蛋白表达与不良预后显著相关（P < 0.050）。综上所述，这些观察结果表明MMP13蛋白过表达可被视为OSCC的预后标志物。
