Singh Shankar Sharan, Bhatt Madan Lal Brahma, Kushwaha Vandana Singh, Singh Anshuman, Kumar Rajendra, Gupta Rajeev, Parmar Devendra
Department of Radiotherapy, King George's Medical University, Lucknow, Uttar Pradesh, India.
Division of Developmental Toxicology, CSIR Indian Institute of Toxicology Research, Lucknow, Uttar Pradesh, India.
J Carcinog. 2017 Jun 19;16:2. doi: 10.4103/jcar.JCar_5_16. eCollection 2017.
Matrix Metalloproteinase 13 (MMP13) is a member of collagenase family and it is involved in the degradation of extracellular matrix and basement membrane protein. It is thought to be associated with tumor invasion and metastasis. Elevated MMP13 expression has been found in carcinoma of the breast, urinary bladder, head and neck and others. It is observed that MMP13 gene is also correlated with radiation response in OSCC (Oral squamous cell carcinoma) cell line based study. The present study correlates the MMP13 expressions with clinicopathological parameters and radiation response in OSCC patients.
The MMP13 mRNA levels were determined by employing qRT-PCR (real-time quantitative reverse transcriptase-polymerase chain reaction).
We observed high expression of MMP13 mRNA in OSCC patients when compared with matched controls. Statistically significant up regulation of MMP13 mRNA expression was found in tobacco chewers, advanced T-stage (p < 0.001) and lymph node metastasis (p < 0.01). MMP13 mRNA levels were also elevated in non responders as compared to responders to radiation treatment.
To the best of our knowledge, this is the first report that indicates role of MMP13 in radiation response in OSCC patients and could be used as potential bio-marker for radiotherapy treatment in OSCC patients.
基质金属蛋白酶13（MMP13）是胶原酶家族的成员，参与细胞外基质和基底膜蛋白的降解。它被认为与肿瘤侵袭和转移有关。在乳腺癌、膀胱癌、头颈癌等肿瘤中发现MMP13表达升高。基于口腔鳞状细胞癌（OSCC）细胞系的研究观察到MMP13基因也与放射反应相关。本研究将MMP13表达与OSCC患者的临床病理参数及放射反应相关联。
采用实时定量逆转录聚合酶链反应（qRT-PCR）测定MMP13 mRNA水平。
与配对对照相比，我们观察到OSCC患者中MMP13 mRNA高表达。在咀嚼烟草者、晚期T分期（p < 0.001）和有淋巴结转移（p < 0.01）的患者中发现MMP13 mRNA表达有统计学意义的上调。与放射治疗有反应者相比，无反应者的MMP13 mRNA水平也升高。
据我们所知，这是第一份表明MMP13在OSCC患者放射反应中的作用的报告，并且可作为OSCC患者放射治疗的潜在生物标志物。
