Cui Hu-Shan, Hayasaka Seiji, Zhang Xue-Yun, Hayasaka Yoriko, Chi Zai-Long, Zheng Lian-Shun
Department of Ophthalmology, Toyama Medical and Pharmaceutical University, Toyama, Japan.
Ophthalmic Res. 2006;38(3):149-57. doi: 10.1159/000090672. Epub 2006 Jan 2.
The aims of this study were to examine the effects of berberine, an alkaloid isolated from some medicinal herbs, on interleukin 8 (IL-8) and monocyte chemotactic protein 1 (MCP-1) expression in a human retinal pigment epithelial cell line (ARPE-19) stimulated with interleukin 1beta (IL-1beta) or tumor necrosis factor alpha (TNF-alpha).
ARPE-19 cells were cultured to confluence. Berberine and IL-1beta or TNF-alpha were added to the medium. IL-8 mRNA and MCP-1 mRNA were measured by semiquantitative reverse-transcription polymerase chain reaction and real-time polymerase chain reaction. IL-8 and MCP-1 protein concentrations in the media were measured using enzyme-linked immunosorbent assay.
Berberine dose-dependently inhibited IL-8 mRNA and MCP-1 mRNA expression of the cells and protein levels in the media stimulated with IL-1beta or TNF-alpha.
These findings indicate that berberine dose-dependently inhibited the expression of IL-8 and MCP-1 induced by IL-1beta or TNF-alpha.
本研究旨在探讨从某些草药中分离出的生物碱黄连素对白细胞介素1β（IL-1β）或肿瘤坏死因子α（TNF-α）刺激的人视网膜色素上皮细胞系（ARPE-19）中白细胞介素8（IL-8）和单核细胞趋化蛋白1（MCP-1）表达的影响。
将ARPE-19细胞培养至汇合状态。向培养基中加入黄连素以及IL-1β或TNF-α。通过半定量逆转录聚合酶链反应和实时聚合酶链反应测量IL-8 mRNA和MCP-1 mRNA。使用酶联免疫吸附测定法测量培养基中IL-8和MCP-1蛋白浓度。
黄连素剂量依赖性地抑制IL-1β或TNF-α刺激的细胞中IL-8 mRNA和MCP-1 mRNA表达以及培养基中的蛋白水平。
这些发现表明黄连素剂量依赖性地抑制IL-1β或TNF-α诱导的IL-8和MCP-1表达。
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