小檗碱对人视网膜色素上皮细胞系屏障功能的影响。
Effect of berberine on barrier function in a human retinal pigment epithelial cell line.
作者信息
Cui Hu-Shan, Hayasaka Seiji, Zhang Xue-Yun, Hayasaka Yoriko, Chi Zai-Long, Zheng Lian-Shun
机构信息
Department of Ophthalmology, University of Toyama, Toyama, Japan.
出版信息
Jpn J Ophthalmol. 2007 Jan-Feb;51(1):64-7. doi: 10.1007/s10384-006-0395-0. Epub 2007 Feb 9.
PURPOSE
To examine the effects of berberine, an alkaloid isolated from some medicinal herbs, on the disruption of the barrier function in a human retinal pigment epithelial cell line (ARPE-19) stimulated with interleukin-1beta (IL-1beta).
METHODS
ARPE-19 cells were cultured to confluence. Berberine and IL-1beta were added to the medium. Barrier functions were evaluated by measuring transepithelial electrical resistance (TER) and the permeability to horseradish peroxidase (HRP) and sodium fluorescein (SF).
RESULTS
Berberine dose-dependently inhibited decreased TER and increased the permeability to HRP and SF in the cells stimulated with IL-1beta.
CONCLUSIONS
Berberine dose-dependently inhibited the disruption of the barrier function in the ARPE-19 cell line induced by IL-1beta.
目的
研究从某些草药中分离出的生物碱黄连素对白细胞介素-1β（IL-1β）刺激的人视网膜色素上皮细胞系（ARPE-19）屏障功能破坏的影响。
方法
将ARPE-19细胞培养至汇合。向培养基中加入黄连素和IL-1β。通过测量跨上皮电阻（TER）以及对辣根过氧化物酶（HRP）和荧光素钠（SF）的通透性来评估屏障功能。
结果
黄连素剂量依赖性地抑制了IL-1β刺激的细胞中TER的降低，并增加了对HRP和SF的通透性。
结论
黄连素剂量依赖性地抑制了IL-1β诱导的ARPE-19细胞系屏障功能的破坏。
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