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小檗碱对脂多糖诱导的人视网膜色素上皮细胞系中单核细胞趋化蛋白-1和白细胞介素-8表达的影响。

Effect of berberine on lipopolysaccharide-induced monocyte chemotactic protein-1 and interleukin-8 expression in a human retinal pigment epithelial cell line.

作者信息

Cui Hu-Shan, Li Yu-Min, Fang Wei, Li Jiu-Ke, Dai Yuan-Min, Zheng Lian-Shun

机构信息

Department of Ophthalmology, Affiliated Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, No. 3 Qingchun Road East, Hangzhou, 310016, Zhejiang Province, China.

Institute of Anatomy and Cell Biology, School of Medicine, Zhejiang University, Hangzhou, China.

出版信息

Int Ophthalmol. 2018 Oct;38(5):2053-2060. doi: 10.1007/s10792-017-0697-x. Epub 2017 Aug 29.

DOI:10.1007/s10792-017-0697-x
PMID:28852897
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6153896/
Abstract

PURPOSE

In this study, we elucidated the effects of berberine, a major alkaloid component contained in medicinal herbs, such as Phellodendri Cortex and Coptidis Rhizoma, on expression of monocyte chemotactic protein-1 (MCP-1) and interleukin-8 (IL-8) in a human retinal pigment epithelial cell line (ARPE-19) caused by lipopolysaccharide (LPS) stimulation.

METHODS

ARPE-19 cells were cultured to confluence. Berberine and LPS were added to the medium. MCP-1 and IL-8 mRNA were measured by real-time polymerase chain reaction. MCP-1 and IL-8 protein concentrations in the media were measured using enzyme-linked immunosorbent assay.

RESULTS

After stimulation with LPS, MCP-1 and IL-8 mRNA in ARPE-19 cells reached maximum levels at 3 h, and MCP-1 and IL-8 protein in the culture media reached maximum levels at 24 h. Berberine dose-dependently inhibited MCP-1 and IL-8 mRNA expression of the cells and protein levels in the media stimulated with LPS.

CONCLUSIONS

These findings indicate that berberine inhibited the expression of MCP-1 and IL-8 induced by LPS.

摘要

目的

在本研究中，我们阐明了黄连素（一种存在于黄柏和黄连等草药中的主要生物碱成分）对脂多糖（LPS）刺激的人视网膜色素上皮细胞系（ARPE - 19）中单核细胞趋化蛋白 - 1（MCP - 1）和白细胞介素 - 8（IL - 8）表达的影响。

方法

将ARPE - 19细胞培养至汇合状态。向培养基中添加黄连素和LPS。通过实时聚合酶链反应测量MCP - 1和IL - 8 mRNA。使用酶联免疫吸附测定法测量培养基中MCP - 1和IL - 8蛋白浓度。

结果

用LPS刺激后，ARPE - 19细胞中的MCP - 1和IL - 8 mRNA在3小时达到最高水平，培养基中的MCP - 1和IL - 8蛋白在24小时达到最高水平。黄连素剂量依赖性地抑制LPS刺激的细胞中MCP - 1和IL - 8 mRNA表达以及培养基中的蛋白水平。

结论

这些发现表明黄连素抑制LPS诱导的MCP - 1和IL - 8表达。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8748/6153896/126fc191e1ce/10792_2017_697_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8748/6153896/ebc35c6c73fd/10792_2017_697_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8748/6153896/bb9b0a9ec9a0/10792_2017_697_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8748/6153896/b641284f8903/10792_2017_697_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8748/6153896/3c35d2983cf8/10792_2017_697_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8748/6153896/f8ba318cd4fb/10792_2017_697_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8748/6153896/126fc191e1ce/10792_2017_697_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8748/6153896/ebc35c6c73fd/10792_2017_697_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8748/6153896/bb9b0a9ec9a0/10792_2017_697_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8748/6153896/b641284f8903/10792_2017_697_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8748/6153896/3c35d2983cf8/10792_2017_697_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8748/6153896/f8ba318cd4fb/10792_2017_697_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8748/6153896/126fc191e1ce/10792_2017_697_Fig6_HTML.jpg

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