应用鼠类探针通过光谱核型分析和多色带技术对小鼠细胞系WMP2进行分子细胞遗传学特征分析。

Molecular cytogenetic characterization of the mouse cell line WMP2 by spectral karyotyping and multicolor banding applying murine probes.

作者信息

Karst Constanze, Trifonov Vladimir, Romanenko Svetlana A, Claussen Uwe, Mrasek Kristin, Michel Susanne, Avner Philip, Liehr Thomas

机构信息

Institute of Human Genetics and Anthropology, D-07743 Jena, Germany.

出版信息

Int J Mol Med. 2006 Feb;17(2):209-13.

PMID:16391817

Abstract

The Moloney murine leukemia virus-transformed suspension cell line WMP2 is derived from wild mice (Mus musculus) of the WMP/WMP strain. These mice carry nine pairs of metacentric Robertsonian translocation chromosomes. As the chromosomes of the wild-type mouse are all acrocentric, metaphase spreads of the WMP2 cells seam to be highly suited for physical gene mapping. Here we studied the WMP2 line using spectral karyotyping (SKY) combined with new established mouse specific multicolor banding (mcb) probes for the chromosomes X, 3, 4, 6 and 18. SKY revealed that the WMP2 cell line developed further four derivative chromosomes. After application of mcb five previously unrecognizable intrachromosomal rearrangements with 9 breakpoints were detected for the studied chromosomes.

摘要

莫洛尼鼠白血病病毒转化的悬浮细胞系WMP2源自WMP/WMP品系的野生小鼠（小家鼠）。这些小鼠携带9对中着丝粒罗伯逊易位染色体。由于野生型小鼠的染色体均为近端着丝粒染色体，WMP2细胞的中期染色体铺展似乎非常适合进行物理基因定位。在此，我们使用光谱核型分析（SKY）结合新建立的针对X、3、4、6和18号染色体的小鼠特异性多色带（mcb）探针研究了WMP2细胞系。SKY显示WMP2细胞系又形成了4条衍生染色体。应用mcb后，在研究的染色体上检测到5个先前未识别的具有9个断点的染色体内重排。