Rudolph Cornelia, Schlegelberger Brigitte
Institute of Cell and Molecular Pathology, Hannover Medical School, Hannover, Germany.
Methods Mol Biol. 2009;506:453-66. doi: 10.1007/978-1-59745-409-4_30.
Cytogenetic characterization of murine chromosomes using banding techniques like R- or G-banding is technically demanding due to the similar size and the acrocentric structure of all chromosomes. The molecular cytogenetic technique of spectral karyotyping (SKY) overcomes that difficulty by karyotyping metaphase chromosomes after different and simultaneous fluorescence labeling of the whole genome. SKY allows the detection and identification of numerical as well as structural chromosome aberrations with a resolution of approximately 2 Mb. The technique is applicable to all fast-proliferating cells, e.g., cells of the hematopoietic system like stem cells or T- and B-lymphocytes. It is also applicable to murine embryonic fibroblast or cells isolated from tissues with increased proliferation-especially tumor tissues. Furthermore, SKY is recommended for the cytogenetic characterization of newly established cell lines.
由于所有小鼠染色体大小相似且均为近端着丝粒结构，使用R显带或G显带等显带技术对小鼠染色体进行细胞遗传学特征分析在技术上要求很高。光谱核型分析（SKY）这一分子细胞遗传学技术通过对整个基因组进行不同的同时荧光标记后对中期染色体进行核型分析，克服了这一困难。SKY能够检测和识别数目及结构染色体畸变，分辨率约为2 Mb。该技术适用于所有快速增殖细胞，例如造血系统细胞，如干细胞、T淋巴细胞和B淋巴细胞。它也适用于小鼠胚胎成纤维细胞或从增殖增加的组织（尤其是肿瘤组织）中分离的细胞。此外，SKY推荐用于新建立细胞系的细胞遗传学特征分析。
