Kumazawa T, Sato K, Seno H, Suzuki O
Department of Legal Medicine, Hamamatsu University School of Medicine, Japan.
Forensic Sci Int. 1992 May;54(2):159-66. doi: 10.1016/0379-0738(92)90160-x.
A simple and rapid method, for the isolation of eight triazine herbicides from human serum and urine, using Sep-Pak C18 cartridges is presented. After mixing with distilled water, serum and urine samples containing the herbicides, were loaded on Sep-Pak C18 cartridges and eluted with either chloroform only or chloroform/methanol (9:1). The herbicides were detected by capillary gas chromatography with both flame ionization detection (FID) and nitrogen-phosphorus detection (NPD). Separation of eight triazine herbicides from each other and from impurities was generally satisfactory with the use of a non-polar DB-1 capillary column. Recovery of most compounds was excellent for both chloroform and chloroform/methanol (9:1) as elution solvents. Backgrounds were cleaner and evaporation time was shorter for the chloroform only than for the chloroform/methanol (9:1). The NPD gave sensitivity more than 10-20 times higher than that of FID.
本文介绍了一种简单快速的方法，使用Sep-Pak C18柱从人血清和尿液中分离出八种三嗪类除草剂。将含有除草剂的血清和尿液样品与蒸馏水混合后，加载到Sep-Pak C18柱上，并用仅氯仿或氯仿/甲醇（9:1）洗脱。通过带有火焰离子化检测（FID）和氮磷检测（NPD）的毛细管气相色谱法检测除草剂。使用非极性DB-1毛细管柱时，八种三嗪类除草剂彼此之间以及与杂质的分离通常令人满意。对于氯仿和氯仿/甲醇（9:1）作为洗脱溶剂，大多数化合物的回收率都很高。仅氯仿的背景比氯仿/甲醇（9:1）更干净，蒸发时间更短。NPD的灵敏度比FID高10-20倍以上。
