Liu J, Suzuki O, Kumazawa T, Seno H
Department of Legal Medicine, Hamamatsu University School of Medicine, Japan.
Forensic Sci Int. 1989 Apr-May;41(1-2):67-72. doi: 10.1016/0379-0738(89)90237-5.
A simple and rapid method for isolation of eleven organophosphate pesticides with Sep-Pak C18 cartridges from human urine and plasma, is presented. The detection of the pesticides was made by wide-bore capillary gas chromatography (GC) with flame ionization detection. The pesticide-containing samples, after mixing with water, were directly applied to the cartridges and eluted with chloroform/isopropanol (9:1). The recoveries with use of the cartridges were excellent for most pesticides. Separation of each pesticide peak from each other and from impurities on the gas chromatograms was also satisfactory with use of non-polar and slightly polar capillary columns. The isolation method with use of the cartridges, together with the wide-bore capillary GC, seems very useful in forensic and environmental chemistry and clinical toxicology.
本文介绍了一种使用Sep-Pak C18柱从人尿和血浆中分离11种有机磷农药的简单快速方法。通过宽口径毛细管气相色谱法（GC）和火焰离子化检测法对农药进行检测。含农药的样品与水混合后，直接加到柱上，并用氯仿/异丙醇（9:1）洗脱。对于大多数农药，使用该柱的回收率极佳。使用非极性和弱极性毛细管柱时，气相色谱图上各农药峰之间以及与杂质之间的分离效果也令人满意。使用该柱的分离方法与宽口径毛细管GC相结合，在法医、环境化学和临床毒理学中似乎非常有用。
