Bioulac-Sage P, Dubuisson L, Bedin C, Gonzalez P, de Tinguy-Moreaud E, Garcin H, Balabaud C
Laboratoire des Interactions Cellulaires, Université de Bordeaux II, France.
Hepatology. 1992 Aug;16(2):418-25. doi: 10.1002/hep.1840160221.
Weaned male rats were fed a 4 ppm selenium diet. Compared after 2 mo with a control group fed a 0.4 ppm diet, the rats' body weights had not significantly decreased and liver function was normal, but portal pressure was 1.8 times higher (p less than 0.05). Liver weight was slightly increased (10.3%; p less than 0.05). All livers had an abnormal appearance. In the less severe cases the surface was only slightly irregular, but in the more severe cases, atrophic micronodular lobes and hypertrophic lobes, with mildly irregular surfaces, were present. On light microscopy, atrophic lobes displayed a peripheral nodular zone with micronodules separated by rows of atrophic hepatocytes without fibrosis, characteristic of nodular regenerative hyperplasia, and a central atrophic zone that was sometimes peliotic. Hypertrophic lobes and livers in the less severe cases had only minor and relatively localized evidence of nodular regenerative hyperplasia; occasional peliosis was seen. In all cases portal veins, hepatic veins and hepatic arteries were normal. By electron microscopy, in nonnodular zones with no obvious evidence of parenchymal atrophy, the endothelial wall showed signs of complete or incomplete capillarization with frequent enlargement of the Disse space. The selenium-enriched diet is a reproducible model of liver nodular regenerative hyperplasia. In this model, damage to the sinusoidal wall could represent the primum movens of microcirculatory disturbances.
将断奶雄性大鼠喂食含4 ppm硒的饮食。2个月后与喂食0.4 ppm饮食的对照组相比，大鼠体重没有显著下降，肝功能正常，但门静脉压力高出1.8倍（p<0.05）。肝脏重量略有增加（10.3%；p<0.05）。所有肝脏外观均异常。在较轻的病例中，表面仅略有不规则，但在较严重的病例中，出现了萎缩性小结节叶和肥大叶，表面轻度不规则。在光学显微镜下，萎缩叶显示出一个周边结节区，有由一排排萎缩肝细胞分隔的小结节，无纤维化，这是结节性再生性增生的特征，还有一个中央萎缩区，有时呈血湖样。肥大叶和较轻病例中的肝脏仅有轻微且相对局限的结节性再生性增生证据；偶尔可见血湖形成。在所有病例中，门静脉、肝静脉和肝动脉均正常。通过电子显微镜观察，在没有明显实质萎缩证据的非结节区，内皮壁显示出完全或不完全毛细血管化的迹象，狄氏间隙频繁扩大。富硒饮食是肝脏结节性再生性增生的一个可重复模型。在这个模型中，窦壁损伤可能代表微循环紊乱的首要因素。
