Marchuk D A, Tavakkol R, Wallace M R, Brownstein B H, Taillon-Miller P, Fong C T, Legius E, Andersen L B, Glover T W, Collins F S
Department of Human Genetics, University of Michigan, Ann Arbor 48109.
Genomics. 1992 Jul;13(3):672-80. doi: 10.1016/0888-7543(92)90140-n.
The yeast artificial chromosome (YAC) system (Burke et al., 1987, Science 236: 806-812) allows the direct cloning of large regions of the genome. A YAC contig map of approximately 700 kb encompassing the region surrounding the type 1 neurofibromatosis (NF1) locus on 17q11.2 has been constructed. A single YAC containing the entire NF1 locus has been constructed by homologous recombination in yeast. In the process of contig construction a novel method of YAC end rescue has been developed by YAC circularization in yeast and plasmid rescue in bacteria. YACs containing homology to the NF1 region but mapping to another chromosome have also been discovered. Sequences of portions of the homologous locus indicate that this other locus is a nonprocessed pseudogene.
酵母人工染色体（YAC）系统（Burke等人，1987年，《科学》236：806 - 812）允许直接克隆基因组的大片段区域。已经构建了一个约700 kb的YAC重叠群图谱，该图谱涵盖了17q11.2上1型神经纤维瘤病（NF1）基因座周围的区域。通过酵母中的同源重组构建了一个包含整个NF1基因座的单一YAC。在重叠群构建过程中，通过酵母中的YAC环化和细菌中的质粒拯救开发了一种新的YAC末端拯救方法。还发现了与NF1区域具有同源性但定位到另一条染色体上的YAC。同源基因座部分的序列表明，这个其他基因座是一个未加工的假基因。
