Rahman Nafisur, Anwar Nishat, Kashif Mohammad
Analytical Research Division, Department of Chemistry, Aligarh Muslim University, India.
Chem Pharm Bull (Tokyo). 2006 Jan;54(1):33-6. doi: 10.1248/cpb.54.33.
A simple and sensitive kinetic spectrophotometric method for the determination of perindopril in pharmaceutical preparations is described. The method is based on the interaction of drug with 1-chloro-2,4-dinitrobenzene (CDNB) in dimethylsulfoxide (DMSO) at 40+/-1 degrees C. The reaction is followed spectrophotometrically by measuring the rate of change of the absorbance at 420 nm. Under the optimized experimental conditions, the calibration curve showed a linear relationship over the concentration range of 20-140 microg/ml. The activation parameter such as E(a), deltaH*, deltaS* and deltaG* for this reaction were calculated and found to be 27.31 kJ/mol, 24.69 kJ/mol, -138.84 J/K/mol and 61.50 kJ/mol, respectively. The method has been successfully applied to the determination of perindopril in commercial dosage forms. Statistical comparison of the results with the Abdellatef's spectrophotometric method shows excellent agreement and indicates no significant difference between the methods compared in terms of accuracy and precision.
本文描述了一种用于测定药物制剂中培哚普利的简单且灵敏的动力学分光光度法。该方法基于药物与1-氯-2,4-二硝基苯（CDNB）在二甲基亚砜（DMSO）中于40±1℃下的相互作用。通过在420nm处测量吸光度的变化速率，采用分光光度法跟踪反应。在优化的实验条件下，校准曲线在20 - 140μg/ml的浓度范围内呈线性关系。计算了该反应的活化参数，如E(a)、ΔH*、ΔS和ΔG，分别为27.31kJ/mol、24.69kJ/mol、-138.84J/K/mol和61.50kJ/mol。该方法已成功应用于商业剂型中培哚普利的测定。将结果与阿卜杜勒泰夫分光光度法进行统计比较，结果显示出极好的一致性，表明在准确度和精密度方面，所比较的方法之间无显著差异。
