Fael Hanan, Sakur Amir Al-Haj
Faculty of Pharmacy, Department of Analytical and Food Chemistry, University of Aleppo, Aleppo, Syrian Arab Republic.
J Fluoresc. 2015 Nov;25(6):1577-84. doi: 10.1007/s10895-015-1666-2. Epub 2015 Oct 5.
A novel, simple and specific spectrofluorimetric method was developed and validated for the determination of perindopril erbumine (PDE). The method is based on the fluorescence quenching of Rhodamine B upon adding perindopril erbumine. The quenched fluorescence was monitored at 578 nm after excitation at 500 nm. The optimization of the reaction conditions such as the solvent, reagent concentration, and reaction time were investigated. Under the optimum conditions, the fluorescence quenching was linear over a concentration range of 1.0-6.0 μg/mL. The proposed method was fully validated and successfully applied to the analysis of perindopril erbumine in pure form and tablets. Statistical comparison of the results obtained by the developed and reference methods revealed no significant differences between the methods compared in terms of accuracy and precision. The method was shown to be highly specific in the presence of indapamide, a diuretic that is commonly combined with perindopril erbumine. The mechanism of rhodamine B quenching was also discussed.
开发并验证了一种新颖、简单且特异的荧光分光光度法用于测定培哚普利叔丁胺盐（PDE）。该方法基于加入培哚普利叔丁胺盐后罗丹明B的荧光猝灭。在500nm激发后，于578nm监测猝灭后的荧光。研究了反应条件如溶剂、试剂浓度和反应时间的优化。在最佳条件下，荧光猝灭在1.0 - 6.0μg/mL浓度范围内呈线性。所提出的方法经过充分验证，并成功应用于纯品和片剂中培哚普利叔丁胺盐的分析。对所开发方法和参考方法所得结果进行统计比较，结果表明在准确性和精密度方面，所比较的方法之间无显著差异。该方法在存在吲达帕胺（一种常与培哚普利叔丁胺盐联用的利尿剂）的情况下显示出高度特异性。还讨论了罗丹明B猝灭的机制。
